First molecular characterization of hypodermin genes of Hypoderma bovis and serodiagnosis of bovine hypodermosis with recombinant hypodermin C antigen and a synthetic peptide containing its linear B-cell epitope
Yazarlar (5)
Dr. Öğr. Üyesi Mübeccel ATELGE Kastamonu Üniversitesi, Türkiye
Abdullah İnci Erciyes Üniversitesi, Türkiye
Alparslan Yıldırım Erciyes Üniversitesi, Türkiye
İbrahim Sözdutmaz Erciyes Üniversitesi, Türkiye
Peter H. Adler Clemson University, Amerika Birleşik Devletleri
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Veterinary Parasitology (Q1) | ||
| Dergi ISSN | 0304-4017 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | Türkçe | Basım Tarihi | 01-2021 |
| Cilt / Sayı / Sayfa | 292 / 1 / 109394–0 | DOI | 10.1016/j.vetpar.2021.109394 |
| Makale Linki | http://dx.doi.org/10.1016/j.vetpar.2021.109394 | ||
| Özet |
| Hypodermins A (HA), B (HB), and C (HC) of warble flies are modulatory antigens involved in host inflammation and immune responses during migration of the warble fly larvae through host connective tissues. In the current study, molecular characteristics of the genes encoding HA, HB, and HC were revealed from cDNA constructs of third-instar larvae of Hypoderma bovis. The open reading frame (ORF) of each hypodermin gene was amplified with modified gene-specific primers, and the resulting PCR products were cloned into pGEM-T Easy Vector to produce recombinant plasmids (rHA, rHB, and rHC). The ORF sequences of rHA, rHB, and rHC genes are 705 bp, 771 bp, and 783 bp long and encode proteins of 234, 256, and 263 amino acids with predicted sizes of 25.74 kDa, 27.79 kDa, and 28.51 kDa, respectively. The rHC gene was subcloned into the pET 100/D-TOPO Expression Vector, and the recombinant … |
| Anahtar Kelimeler |
| Hypoderma bovis | Hypodermin genes | Molecular characterization | Recombinant antigen | Serodiagnosis | Synthetic peptide |
Science Direct
First molecular characterization of hypodermin genes of Hypoderma bovis and serodiagnosis of bovine hypodermosis with recombinant hypodermin C antigen and a synthetic peptide containing its linear B-cell epitope
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Web of Science | 7 |
| Scopus | 9 |
| Google Scholar | 11 |