| Yazarlar |
Prof. Dr. Özgür KAYNAR
Atatürk Üniversitesi, Türkiye |
Mustafa İleritürk
Atatürk Üniversitesi, Türkiye |
|
Armağan Hayırlı
Atatürk Üniversitesi, Türkiye |
| Özet |
| High-performance thin-layer chromatography plays an important role in the determination of lipid fractions. However, it requires expensive equipment for quantification of lipids spots. In this experiment, flatbed scanner with gel analysis software was employed to determine lipid fractions of egg yolk, with testing some modifications: optical resolution (300 vs. 600 dpi), lane selection method (manual vs. automatic), and background subtraction options (none, lane edge, minimum profile, rubber band, rolling ball, or valley to valley). Data show that using flatbed scanner with gel analysis software is economically feasible and yields reliable and reproducible results. The best chromatogram was achieved at 600 dpi resolution with no background subtraction, regardless of lane selection. © 2013 Akadémiai Kiadó, Budapest. |
| Anahtar Kelimeler |
| TL 120 software,Flatbed scanner,Visible light,High quality image |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | JPC-JOURNAL OF PLANAR CHROMATOGRAPHY- MODERN TLC |
| Dergi ISSN | 0933-4173 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Makale Dili | İngilizce |
| Basım Tarihi | 01-2013 |
| Cilt No | 26 |
| Sayı | 3 |
| Sayfalar | 202 / 208 |
