Evaluation Of Computational Modifications In HPTLC With Gel Analysis Software And Flatbed Scanner For Lipid Separation
Yazarlar (3)
Prof. Dr. Özgür KAYNAR Atatürk Üniversitesi, Türkiye
Doç. Dr. Mustafa İleritürk Atatürk Üniversitesi, Türkiye
Armağan Hayırlı
Atatürk Üniversitesi, Türkiye
Makale Türü Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
Dergi Adı Journal of Planar Chromatography Modern TLC
Dergi ISSN 0933-4173 Wos Dergi Scopus Dergi
Dergi Tarandığı Indeksler SCI-Expanded
Makale Dili İngilizce Basım Tarihi 01-2013
Cilt / Sayı / Sayfa 26 / 3 / 202–208 DOI 10.1556/JPC.26.2013.3.1
Özet
High-performance thin-layer chromatography plays an important role in the determination of lipid fractions. However, it requires expensive equipment for quantification of lipids spots. In this experiment, flatbed scanner with gel analysis software was employed to determine lipid fractions of egg yolk, with testing some modifications: optical resolution (300 vs. 600 dpi), lane selection method (manual vs. automatic), and background subtraction options (none, lane edge, minimum profile, rubber band, rolling ball, or valley to valley). Data show that using flatbed scanner with gel analysis software is economically feasible and yields reliable and reproducible results. The best chromatogram was achieved at 600 dpi resolution with no background subtraction, regardless of lane selection. © 2013 Akadémiai Kiadó, Budapest.
Anahtar Kelimeler
Flatbed scanner | High quality image | TL 120 software | Visible light