Evaluation of cardiac troponin I in serum and myocardium of rabbits with experimentally induced polymicrobial sepsis
Yazarlar (3)
Doç. Dr. Kenan Çağrı TÜMER Fırat Üniversitesi, Türkiye
Prof. Dr. Haydar Özdemir Fırat Üniversitesi, Türkiye
Prof. Dr. Hatice Eröksüz Fırat Üniversitesi, Türkiye
| Makale Türü |
Özgün Makale
(SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
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| Dergi Adı | Experimental Animals (Q1) | ||
| Dergi ISSN | 1341-1357 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 01-2020 |
| Cilt / Sayı / Sayfa | 69 / 1 / 54–61 | DOI | 10.1538/expanim.19-0046 |
| Makale Linki | https://www.jstage.jst.go.jp/article/expanim/advpub/0/advpub_19-0046/_article | ||
| Özet |
| Sepsis is a potentially life-threatening condition, and it is frequently complicated by myocardial damage. Data on myocardial damage in rabbit caecal ligation and puncture (CLP) models are limited, although numerous animal models have been used to study sepsis-associated myocardial damage. This study aimed to investigate the effect of CLP on cardiac muscle by measuring serum cardiac troponin I (cTnI) concentrations and by detecting both histopathological changes and cTnI immunoreactivity in cardiomyocytes in rabbits. After CLP was performed in rabbits, blood samples were taken from the jugular vein at 0, 4, 8, and 12 h for haematological and biochemical analyses. At the end of the experiment, all of the rabbits were euthanised to examine the histopathological changes and the cTnI immunoreactivity in cardiac muscle tissue. No changes in serum cTnI concentration were observed in the experimental group (EG) or control group (CG) at 0 and 4 h. In EG, the mean serum cTnI concentrations were 0.230±0.209 and 1.177±0.971 ng/ml at 8 and 12 h, respectively. In CG, the mean serum cTnI concentrations were 0.032±0.014 and 0.031±0.021 ng/ml at 8 and 12 h, respectively. Moreover, cytoplasmic cTnI immunoreactivity decreased in EG compared with that in CG (P< 0.01). The results demonstrated that CLP induced a systemic inflammatory response and caused myocardial damage in rabbits. |
| Anahtar Kelimeler |
| Caecal ligation and puncture | Cardiac troponin I | Immunohistochemistry | Rabbit | Sepsis |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Web of Science | 9 |
| Scopus | 10 |
| Google Scholar | 15 |