| Yazarlar |
Dr. Öğr. Üyesi Hakan KÜÇÜKSAYAN
Kastamonu Üniversitesi, Türkiye |
Şakir Akgün
Türkiye |
|
Osman Nidai Özeş
Türkiye |
|
Mustafa Yıldız
Türkiye |
|
Arsenal Sezgin Alikanoğlu
Türkiye |
|
Egemen Dal
|
|
Hakan Akça
Pamukkale Üniversitesi, Türkiye |
| Özet |
| Transforming growth factor-β (TGF-β) pathway plays crucial roles during the carcinogenesis and metastasis. TGF-β receptor 2 (TGFBR2) is a key molecule for the regulation of TGF-β pathway and frequently downregulated or lost in several cancer types including non-small cell lung cancer (NSCLC), and TGF-β pathway is often regulated by negative-feedback mechanisms, but little is known about the mechanism of TGFBR2 downregulation in NSCLC. Here, we found that the expression of miR-520e is upregulated in metastatic tumor tissues compared with non-metastatic ones, and its expression is inversely correlated with that of TGFBR2 in clinical samples. We also discovered that TGF-β dramatically increased the expression of miR-520e, which targeted and downregulated TGFBR2, and the suppression of miR-520e significantly impaired TGF-β-induced TGFBR2 downregulation. Chromatin immunoprecipitation-PCR experiments further showed that miR-520e is transcriptionally induced by SMAD2/3 in response to TGF-β. Our findings reveal a novel negative-feedback mechanism in TGF-β signaling and the expression level of miR-520e could be a predictive biomarker for NSCLC metastasis. |
| Anahtar Kelimeler |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | Carcinogenesis |
| Dergi ISSN | 0143-3334 |
| Dergi Tarandığı Indeksler | SCI |
| Makale Dili | Türkçe |
| Basım Tarihi | 11-2018 |
| Cilt No | 40 |
| Sayı | 5 |
| Sayfalar | 695 / 705 |
| Doi Numarası | 10.1093/carcin/bgy166 |
| Makale Linki | http://dx.doi.org/10.1093/carcin/bgy166 |
| Atıf Sayıları | |
| WoS | 9 |
