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Histopathological, immunohistochemical and biochemical alterations in liver tissue after fungicide-mancozeb exposures in Wistar albino rats       
Yazarlar
Doç. Dr. Ertuğrul GÖK
Dicle Üniversitesi, Türkiye
Engin Deveci
Dicle Üniversitesi, Türkiye
Özet
Purpose: To evaluate the histopathological, immunohistochemical, and biochemical effects of liver changes after mancozeb administration. Methods: Rats were divided into groups–the control group (n=7) and the mancozeb group (n=7)–, given 500 mg/kg mancozeb dissolved in corn oil daily for four weeks by an orogastric tube. Caspase-3 and tumor necrosis factor-alpha (TNF-α) primary antibodies were used for immunohistochemical analysis. Results: Serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) values of the mancozeb group increased significantly than ones of the control group. Venous dilatation, inflammation, hepatocyte degeneration, TNF-α, and caspase-3 expression scores increased significantly in the mancozeb group. In the mancozeb group, intensive caspase-3 expression was observed in hepatocyte cells around the central vein in the center of the liver lobule, and there was an increase in TNF-α expression in the inflammatory cells around the enlarged central vein and Kupffer cells and apoptotic hepatocyte cells. Conclusion: Subacute mancozeb exposure in rats leads to elevated toxicity with impaired liver function, increased inflammation in tissue and increased apoptosis due to cellular damage in the liver, and decreased liver regeneration ability due to congestion and degeneration of blood vessels.
Anahtar Kelimeler
Immunohistochemistry | Liver | Toxicity
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale
Dergi Adı ACTA CIRURGICA BRASILEIRA
Dergi ISSN 0102-8650
Dergi Tarandığı Indeksler SCI-Expanded
Dergi Grubu Q4
Makale Dili İngilizce
Basım Tarihi 01-2022
Cilt No 37
Sayı 4
Doi Numarası 10.1590/acb370404
Makale Linki https://www.scielo.br/j/acb/a/4gn7rRSzd3Dy7nB4DNLG8Pf/?format=pdf&lang=en