| Yazarlar |
Prof. Dr. Ergin Murat ALTUNER
Kastamonu Üniversitesi, Türkiye |
Prof. Dr. Talip ÇETER
Kastamonu Üniversitesi, Türkiye |
Hami Alpas
Orta Doğu Teknik Üniversitesi, Türkiye |
| Özet |
| High hydrostatic pressure (HHP) has high success potential in pollen protein extraction, but its effect on pollen protein profiles has not been studied yet. The aim of this study is to put forward whether HHP processing causes a change in the protein profiles extracted from pollens or not. In this study, proteins extracted from Betula pendula pollens were studied at 100, 200 and 300MPa at room temperature for 5min. In addition, the efficiency of three different extraction solvents, namely phosphate buffer saline (PBS) buffer pH 7.5, trichloroacetic acid-acetone and Tris-HCl buffer pH 8.8, was also observed, and the results were compared with the conventional pollen protein extraction procedure. As a result, it is concluded that 200MPa for 5min has extracted similar amounts of protein compared with the conventional extraction method which lasted for 24h, which lasted for 24 h. On the other hand, the application time for 200 MPa for 5 min is extremely shorter when it is compared to the conventional extraction method. |
| Anahtar Kelimeler |
| Betula pendula | high hydrostatic pressure | pollen proteins | protein extraction | protein profiles | silver birch |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | HIGH PRESSURE RESEARCH |
| Dergi ISSN | 0895-7959 |
| Dergi Tarandığı Indeksler | SCI-Exp, SCOPUS, Curation, Current Contents Physical Chemical & Earth Sciences, Essential Science Indicators, Reference Master, Sophia |
| Dergi Grubu | Q3 |
| Makale Dili | İngilizce |
| Basım Tarihi | 10-2014 |
| Cilt No | 34 |
| Sayı | 4 |
| Sayfalar | 470 / 481 |
| Doi Numarası | 10.1080/08957959.2014.990142 |
| Atıf Sayıları | |
| SCOPUS | 3 |
| Google Scholar | 7 |
