The value of PCR method in the species-level identification of a mucoid Salmonella sp. Strain isolated from the urine culture of a case with asymptomatic nephrolithiasis
Yazarlar (4)
Fulya Bayindir Bilman Diyarbakir Training And Research Hospital, Türkiye
Doç. Dr. Elçin GÜNAYDIN Etlik Veterinary Control Center Institute, Türkiye
Mine Turhanoǧlu Diyarbakir Training And Research Hospital, Türkiye
Ali Akkoç Diyarbakir Training And Research Hospital, Türkiye
| Makale Türü | Özgün Makale (SCOPUS dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Mikrobiyoloji Bulteni | ||
| Dergi ISSN | 0374-9096 Wos Dergi Scopus Dergi | ||
| Makale Dili | İngilizce | Basım Tarihi | 01-2014 |
| Cilt / Sayı / Sayfa | 48 / 1 / 151–159 | DOI | – |
| Makale Linki | https://www.researchgate.net/profile/Elcin_Gunaydin/publication/260128563_The_value_of_PCR_method_in_the_species-level_identification_of_a_mucoid_Salmonella_sp_strain_isolated_from_the_urine_culture_of_a_case_with_asymptomatic_nephrolithiasis/links/5 | ||
| Özet |
| Colonies of the Salmonella strains usually show a smooth (S) character. Therefore, Salmonella strains a producing mucoid colony are very rarely encountered in the literature. Identification of the mucoid Salmonella strains to the species level is difficult via conventional methods, since the mucus layer does a not allow the bacterium to respond to the antigenic reactions. In this study we aimed to emphasize the identification of Salmonella serotypes by the polymerase chain reaction (PCR) when rough (R) or mucoid a (M) Salmonella isolates are encountered in the laboratory. The urine culture of a 17-year-old female a patient revealed growth of 100.000 cfu/mL gram-negative bacilli in mucoid colony morphology. The isolate was identified as Salmonella sp. by biochemical tests and Vitek 2 (bioMérieux, France) automated a identification system. Agglutination tests showed negative reaction with the known antiserums. Absence of agglutination was attributed to the mucoid character of the isolate. Identification of the Salmonella sp. a was confirmed by Vitek MS MALDI-TOF (bioMérieux, France) analysis method, however, the serotype of the strain could not be identified. In order to verify that the mucoid colony was Salmonella spp., a species-specific PCR was performed using invA primers, and Salmonella sp. identification was verified by observing a 284 base-pair (bp) PCR amplicon. Subsequently, serogrouping was done by multiplex-PCR (mPCR), which could identify the O: B (O: 4), O: C1 (O: 7), O: C2-C3 (O: 8), O: D (O: 9, O: 9, 46, O: 9, 46, 27), and O: E (O: 3, 10, O: 3, 19) somatic antigens. It was detected that the mucoid Salmonella sp. formed a … |
| Anahtar Kelimeler |
| Mucoid salmonella | Polymerase chain reaction | Salmonella enteritidis |
