Tissue-Protective Effects of French Maritime Pine Bark (Pycnogenol) on Glutamate-induced Cytotoxicity in Adult Humanderma Fibroblasts.
Yazarlar (7)
Doç. Dr. Çiğdem SEVİM Kastamonu Üniversitesi, Türkiye
Elif Doğan
Ali Taghizadehghalehjoughi
Semin Gedikli
Mustafa Özkaraca
Selim Çomaklı
Yavuz Selim Sağlam
| Bildiri Türü | Tebliğ/Bildiri | Bildiri Dili | İngilizce |
| Bildiri Alt Türü | |||
| Bildiri Niteliği | Web of Science Kapsamındaki Kongre/Sempozyum | ||
| DOI Numarası | 10.1016/j.toxlet.2017.07.445 | ||
| Kongre Adı | 53rd Congress of the European-Societies-of-Toxicology (EUROTOX) | ||
| Kongre Tarihi | 10-09-2017 / 13-09-2017 | ||
| Basıldığı Ülke | Slovakya | Basıldığı Şehir | |
| Bildiri Linki | https://linkinghub.elsevier.com/retrieve/pii/S0378427417306823 | ||
| UAK Araştırma Alanları |
Tıbbi Farmakoloji
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| Özet |
| French maritime pine bark (Pycnogenol), an extract of pine bark, is known to have cytoprotective, antioxidant, and anti-inflammatory properties. The aim of the present study was to confirm the protective effects of Pycnogenol on glutamate-induced tissue degeneration by using adult human dermal fibroblasts. This study demonstrates that a wide variety of Pycnogenol concentrations inhibit glutamate-induced cytotoxicity in adult human dermal fibroblasts. Different concentrations (1.95–2000 M) of Pycnogenol was highly effective in protecting fibroblastic cells against acute and chronic cytotoxicity. Specifically, we studied the role of oxidative damage as possible mechanisms of glutamate cytotoxicity. Consequently, we have chosen to use the adult human dermal fibroblasts as a model for investigation of glutamate-toxicity and the effect of Pycnogenol on glutamate-exposed cells. In vitro study (GSH, SOD, MDA and cell viability) was performed by using different methods such as biochemical analyzer, ELISA and MTT. Glutamate and Pycnogenol were applied to cells in different doses. Pycnogenol, at increased concentrations after 24 h and 48 h caused significant elevations in cell viability. The treatment doses 1.95–15.63 and 31.25 M Pycnogenol significantly suppressed the glutamate-stimulated upregulation of MDA to the basal levels and increased SOD and GSH in adult human dermal fibroblasts. These findings demonstrate that Pynogenol has a protective role in cytotoxicity caused by Glutamate and Pcynogenol may be useful as adjuvant therapy for tissue disease treatments. http://dx. doi. org/10.1016/j. toxlet. 2017.07. 445 |
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BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Web of Science | 3 |
| Google Scholar | 3 |