| Yazarlar |
Feray Altan
Dokuz Eylül Üniversitesi, Turkey |
|
Orhan Corum
Mustafa Kemal Üniversitesi, Turkey |
|
Duygu Durna Corum
Mustafa Kemal Üniversitesi, Turkey |
|
Kamil Uney
Selçuk Üniversitesi, Turkey |
Doç. Dr. Ertuğrul TERZİ
Kastamonu Üniversitesi, Türkiye |
Doç. Dr. Soner BİLEN
Kastamonu Üniversitesi, Türkiye |
Doç. Dr. Adem Yavuz SÖNMEZ
Kastamonu Üniversitesi, Türkiye |
|
Muammer Elmas
Selçuk Üniversitesi, Turkey |
| Özet |
| Objective: Doxycycline (DO) has been used in fish for a long time, but there are some factors that have not yet been clarified regarding its pharmacokinetic (PK) and pharmacodynamic (PD) properties. Therefore, the aim of this study was to investigate the PK and PK/PD targets of DO after 20 mg/kg intravascular (IV), intramuscular (IM) and oral (OR) gavage administration in rainbow trout (Oncorhynchus mykiss). Methods: Plasma samples were collected at specific time points and subsequently analysed by HPLC-ultraviolet. The PK/PD indices were calculated based on the MIC90 (Aeromonas hydrophila and Aeromonas sobria) values obtained for the respective bacteria and the PK parameters obtained for DO following both IM and OR administration. Results: After IV administration, the elimination half-life (t1/2ʎz), area under the concentration vs. time curve (AUC), apparent volume of distribution at steady-state and total body clearance of DO were 34.81 h, 723.82 h µg/mL, 1.24 L/kg and 0.03 L/kg/h, respectively. The t1/2λz of the DO was found to be 37.39 and 39.78 h after IM, and OR administration, respectively. The bioavailability was calculated 57.02% and 32.29%, respectively, after IM and OR administration. The MIC90 of DO against A. hydrophila and A. sobria was 4 µg/mL. The PK/PD integration showed that DO (20 mg/kg dose) for A. hydrophila and A. sobria with MIC90 ≤4 µg/mL achieved target AUC/MIC value after IM administration. Conclusions: These results suggest that when rainbow trout was treated with 20 mg/kg IV and IM administered DO, therapeutically effective concentrations were reached in the control of infections caused by A. hydrophila and A. sobria. |
| Anahtar Kelimeler |
| Aeromonas hydrophila | Aeromonas sobria | doxycycline | pharmacokinetics | PK–PD integration | rainbow trout |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | Veterinary Medicine and Science |
| Dergi ISSN | 2053-1095 |
| Dergi Grubu | Q2 |
| Makale Dili | İngilizce |
| Basım Tarihi | 05-2024 |
| Cilt No | 10 |
| Sayı | 3 |
| Doi Numarası | 10.1002/vms3.1419 |
| Atıf Sayıları |
