| Yazarlar |
Ahmet Harmankaya
Kafkas Üniversitesi, Turkey |
|
Irfan Çinar
Kastamonu University, Turkey |
|
Muhammed Yayla
Kafkas Üniversitesi, Turkey |
|
Sezen Harmankaya
Kafkas Üniversitesi, Turkey |
|
Murat Beytur
Kafkas Üniversitesi, Turkey |
|
Cem Öziç
Kafkas Üniversitesi, Turkey |
| Özet |
| This study evaluated the effects of extracts prepared using two different methods (decoction extraction and soxhlet ethyl acetate/hexane extraction) from Inula viscosa on H2O2-induced oxidative stress and wound healing model in mouse L929 fibroblast cells. The cytotoxic effect started to disappear statistically (p<0.05) at concentrations of Soxhlet ethyl acetate/hexane extracts (SoxEHE) in and below 0.1 mg/ mL, while the same effect was observed at concentrations of decoction extracts (DE) in and below 0.2 mg/mL. Therefore, 0.2 and 0.02 mg/ mL concentrations of DE, and 0.1 and 0.01 mg/mL concentrations of SoxEHEs were used. While cell migration was positively affected in all research concentrations, statistically significant results (p<0.05) were obtained from 0.2 mg/mL of DE and 0.1 mg/mL of SoxEHE extracts. Malondialdehyde (MDA) levels were found to be statistically (p <0.05) decreased, but COL1A1 levels were higher in cell lines treated with oxidative stress + extract than in the cell line treated only with H2O2, and reduced glutathione (GSH) levels were higher in cell lines only treated only with extract than in oxidative stress-induced cell lines. Consequently, it has been observed that the extracts have positive effects on migration and oxidative stress. Therefore, I. viscosa may serve as a new therapeutic agent for wound healing. |
| Anahtar Kelimeler |
| cell proliferation | extraction | Medicinal plants | oxidative status | scratch assay |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SCOPUS dergilerinde yayımlanan tam makale |
| Dergi Adı | Fabad Journal of Pharmaceutical Sciences |
| Dergi ISSN | 1300-4182 |
| Makale Dili | İngilizce |
| Basım Tarihi | 03-2024 |
| Cilt No | 49 |
| Sayı | 1 |
| Sayfalar | 129 / 142 |
| Doi Numarası | 10.55262/fabadeczacilik.1364029 |
| Atıf Sayıları |
