Expression of circulating oar-miR-485-5p and oar-miR-493-5p during the estrous cycle and early pregnancy in ovine plasma
Yazarlar (4)
Eyyup Hakan Ucar
Doç. Dr. Mustafa Hitit Kastamonu Üniversitesi, Türkiye
Mehmet Kose
Mehmet Osman Atli
| Makale Türü |
Özgün Makale
(SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
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| Dergi Adı | ANIMAL REPRODUCTION (Q2) | ||
| Dergi ISSN | 1806-9614 Dergi Bilgileri (2024) | ||
| Makale Dili | İngilizce | Basım Tarihi | 01-2024 |
| Kabul Tarihi | – | Yayınlanma Tarihi | 01-01-2024 |
| Cilt / Sayı / Sayfa | 21 / 1 / – | DOI | 10.1590/1984-3143-AR2023-0115 |
| Makale Linki | https://www.scielo.br/j/ar/a/Zjwjmn64mBX4xwSc8K7qW7j/?lang=en&format=pdf | ||
| UAK Araştırma Alanları |
Sağlık Bilimleri
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| Özet |
| In the current study, we aimed to assess the expression levels of two circulating microRNAs (miRNA) (oar-miR-485-5p and oar-miR-493-5p) in the ovine plasma during the peri-implantation. After mating, we collected the plasma samples from a total of 8 ewes on day 22 of pregnancy (P22; n = 4) and day 22 of the estrous cycle (C22; n=4). We used mature miRNA sequences for oar-miR-485-5p and oar-miR-493-5p out of one hundred fifty, which were retrieved from our microarray results of previous study. We showed that the miRNA expression of oar-miR-485-5p and oar-miR-493-5p were upregulated in P22 (P<0.05) when compared to C22. Those two miRNAs targeted 311 target genes in the peri-implantation period of pregnancy. Furthermore, we revealed 151 GO/pathway terms in biological process (BP) and 25 GO/pathway terms in molecular function (MF), while we demonstrated 13 GO/pathway terms in cellular component (CC). We revealed three hub genes as interleukin 2 (IL2), interleukin 18 (IL18), and C-X-C Motif Chemokine Ligand 10 (CXCL10). In conclusion, both miR-485-5p and oar-miR-493-5p have the potential to be a biomarker to understand peri-implantation of the ovine pregnancy in the aspect of pregnancy-reflected changes in maternal plasma. |
| Anahtar Kelimeler |
| microRNA | oar-miR-485-5p | oar-miR-493-5p | ovine | plasma |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları |