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Detection of the lactic acid bacterial community in small scale white cheese processing facility using shotgun metagenomics and culturomics      
Yazarlar
Zeynep Görkem Cerit
Türkiye
Özge Kahraman Ilıkkan
Türkiye
Prof. Dr. Mehmet Cengiz BALOĞLU Prof. Dr. Mehmet Cengiz BALOĞLU
Kastamonu Üniversitesi, Türkiye
Remziye Yılmaz
Hacettepe Üniversitesi, Türkiye
Özet
This study aims to characterize lactic acid bacteria (LAB) community in small-scale white cheese processing facility highly favored by consumers. Shotgun metagenomics and culturomics based on MALDI-TOF MS approaches were employed. Samples from various production stages and equipment surfaces were collected. Dominant phyla in dairy and equipment samples were Firmicutes (63–70%) and Proteobacteria (84–89%), respectively. Lactococcus was the most abundant LAB genus across all samples, with Lactococcus lactis as dominant species followed by Streptococcus, Enterococcus, and Lactobacillus, indicating LAB community diversity contributing to product characteristics. Culturomics identified 12 LAB species, including Enterococcus faecalis and Lactobacillus delbrueckii. Metagenomics provided insights into microbial composition and dynamics in dairy environment, while culturomics identified specific LAB species. The combined approach offers a comprehensive view of dairy microbial communities, aiding industrial development by informing starter culture combinations for improved dairy products and processes.
Anahtar Kelimeler
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale
Dergi Adı International Dairy Journal
Dergi ISSN 0958-6946
Dergi Tarandığı Indeksler SCI
Dergi Grubu Q2
Makale Dili Türkçe
Basım Tarihi 08-2024
Cilt No 155
Sayı 105971
Doi Numarası 10.1016/j.idairyj.2024.105971
Makale Linki https://doi.org/10.1016/j.idairyj.2024.105971