Chlorogenic Acid: HPLC Quantification and In Vitro Assessment of Proliferative and Migration Effects on Human Dermal Fibroblast Cells
Yazarlar (6)
Öğr. Gör. Abdulbaki AKPINAR Kastamonu Üniversitesi, Türkiye
Prof. Dr. Fatma Demirkaya Miloğlu Atatürk Üniversitesi, Türkiye
Prof. Dr. Gülşah Gündoğdu Pamukkale Üniversitesi, Türkiye
Doç. Dr. Leyla Güven Atatürk Üniversitesi, Türkiye
Arş. Gör. Burak Bayrak Atatürk Üniversitesi
Prof. Dr. Yücel Kadıoğlu Atatürk Üniversitesi, Türkiye
| Makale Türü |
Özgün Makale
(Ulusal alan endekslerinde (TR Dizin, ULAKBİM) yayınlanan tam makale)
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| Dergi Adı | Cumhuriyet Science Journal | ||
| Dergi ISSN | 2587-2680 | ||
| Dergi Tarandığı Indeksler | TR DİZİN | ||
| Makale Dili | Türkçe | Basım Tarihi | 06-2024 |
| Kabul Tarihi | 21-06-2024 | Yayınlanma Tarihi | 30-06-2024 |
| Cilt / Sayı / Sayfa | 45 / 2 / 299–308 | DOI | 10.17776/csj.1440382 |
| Makale Linki | http://dx.doi.org/10.17776/csj.1440382 | ||
| UAK Araştırma Alanları |
Analitik Kimya
Organik Kimya
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| Özet |
| Chlorogenic acid (CA) exhibits diverse biological activities, including antioxidant and antiinflammatory effects. This research aims to develop, optimize, and validate an HPLC method to quantify CA in methanol and investigate its in vitro proliferative and cell migration effects on human-dermal-fibroblast (HDF) cell lines in a dose-dependent manner. The HPLC experimental conditions were optimized using the central composite design (CCD) method for determining CA. Chromatographic separation occurred at a wavelength of 330 nm. Under the optimized conditions, the method exhibited linearity across a concentration range of 0.1-100 µg/mL, demonstrating sensitivity (LOQ:0.1µg/mL), precision (RSD%≤3.32), and accuracy (RE%≤4.05). To evaluate the in vitro proliferative and cell migration effects on HDFs, we employed the XTT cell proliferation assay and TAS-TOS commercial kits. The XTT assay revealed that CA displayed a proliferative effect within the concentration range of 75-250 µM (P <0.01), and at a concentration of 125 µM, TAS levels increased significantly (P<0.05). The scratch assay demonstrated that HDF cell migration increased at 12 h, with substantial closure of the wound area at 24 h when treated with CA concentrations between 75-125 µM. The results demonstrate that pure chlorogenic acid extracted from plants exhibits dose-dependent effects on cell proliferation, antioxidant, and cell migration |
| Anahtar Kelimeler |
| Chlorogenic acid | HPLC | Experimental design | Central composite design |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Google Scholar | 1 |