| Yazarlar |
Ayhan Vurmaz
Afyon Kocatepe Üniversitesi, Türkiye |
|
Reşat Duman
Afyonkarahisar Sağlık Bilimleri Üniversitesi, Türkiye |
Dr. Öğr. Üyesi Mehmet Cem SABANER
Afyonkarahisar Sağlık Bilimleri Üniversitesi, Türkiye |
|
Tolga Ertekin
Afyonkarahisar Sağlık Bilimleri Üniversitesi, Türkiye |
|
Abdülkadir Bilir
Afyonkarahisar Sağlık Bilimleri Üniversitesi, Türkiye |
| Özet |
| The etiopathogenesis of steroid-induced cataracts is unknown. One hypothesis is that the higher reactive oxygen species (ROS) levels play an important role in the pathogenesis of several disorders, including the evolution of cataracts. This study investigated the antioxidant effects of piperine in our steroid-induced chick embryo lens model. The study included 36 specific pathogen-free (SPF) fertilized eggs divided into six groups: phosphate buffer saline (PBS, pH 7.4 Saline Solution (0.9%) isotonic) group (G1), hydrocortisone succinate sodium (HC)-treated group (G2), 100 mg/kg piperine and HC treated group (G3), 50 mg/kg piperine and HC treated group (G4), 25 mg/kg piperine and HC treated group (G5), and 10 mg/kg piperine and HC treated group (G6). On the 15th day of incubation, the SPF eggs in the six groups were removed from the incubator; all were injected using insulin injectors into the chorioallantoic membrane. On day 17, all of the chick embryos were removed from the eggs and all lenses were dissected from the embryos. Cataract formation was evaluated in all lenses, and total antioxidant status (TAS), total oxidant status (TOS), reduced glutathione (GSH), and lipid peroxidation (MDA, malondialdehyde) levels were measured in all lens. The lenses in the G1 group had higher levels of GSH and TAS (p < 0.01), and lower levels of MDA and TOS than the G2 group (p < 0.05 and p < 0.01, respectively). Group 3 had higher levels of GSH and TAS (p < 0.001 and p < 0.001 respectively), and lower levels of MDA and TOS than the G2 group (p < 0.01 and p < 0.001, respectively). Steroid therapy causes a decrease in GSH and TAS levels and an increase in TOS and MDA levels in lens tissues, indicating increased oxidative stress. Piperine exerts its effects as an antioxidant substance, in increasing doses. |
| Anahtar Kelimeler |
| Cataract | chick | embryo | steroid |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | CUTANEOUS AND OCULAR TOXICOLOGY |
| Dergi ISSN | 1556-9527 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q3 |
| Makale Dili | İngilizce |
| Basım Tarihi | 04-2019 |
| Cilt No | 38 |
| Sayı | 2 |
| Sayfalar | 182 / 189 |
| Doi Numarası | 10.1080/15569527.2019.1570521 |
| Makale Linki | https://www.tandfonline.com/doi/full/10.1080/15569527.2019.1570521 |
| Atıf Sayıları | |
| WoS | 16 |
