Kinetic Behaviour of Glucose 6 Phosphate Dehydrogenase and 6 Phosphogluconate Dehydrogenase in Different Tissues of Rainbow Trout Oncorhynchus mykiss Exposed to Non Lethal Concentrations of Cadmium
Yazarlar (5)
Olcay Hisar
Çanakkale Onsekiz Mart Üniversitesi, Türkiye
Çanakkale Onsekiz Mart Üniversitesi, Türkiye
Doç. Dr. Adem Yavuz SÖNMEZ Kastamonu Üniversitesi, Türkiye
Şükrü Beydemir Atatürk Üniversitesi, Türkiye
Şükriye Aras Hisar Çanakkale Onsekiz Mart Üniversitesi, Türkiye
Tom Cronın
Suny Cobleskill, Amerika Birleşik Devletleri
Suny Cobleskill, Amerika Birleşik Devletleri
| Makale Türü |
Özgün Makale
(SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
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| Dergi Adı | Acta Veterinaria Brno (Q4) | ||
| Dergi ISSN | 0001-7213 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 01-2009 |
| Cilt / Sayı / Sayfa | 78 / 1 / 179–185 | DOI | 10.2754/avb200978010179 |
| Makale Linki | http://actavet.vfu.cz/78/1/0179/ | ||
| Özet |
| The effects of cadmium (Cd) on the enzymatic activities of glucose 6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) were investigated in the gill, liver and kidney tissues of rainbow trout (Oncorhynchus mykiss). Three test groups of fish were subjected to increasing concentrations (1, 3 and 5 mg/l) of cadmium (Cd) in vivo, respectively. The G6PD and 6PGD activities in the gill, liver, and kidney tissues of each group of fish were measured on days 1, 3, 5 and 7. G6PD and 6PGD enzyme activities, measured in gill, liver and kidney homogenates, were stimulated by various concentrations (1, 3, and 5 mg/l) of cadmium. Although the dose-response pattern of G6PD enzyme activities in liver and kidney tissue was very similar, that in gill was different from both other tissues. The enzyme activity of G6PD enzyme was significantly stimulated after three days (Day 3) in liver and kidney tissues at a dose of 1 mg/l Cd (p<0.05), whereas it was stimulated on the first day of experiment (Day 1) in gill, liver and kidney tissues at doses of 3 and 5 mg/l Cd (p<0.05). However, the activity of 6PGD was stimulated after three days (Day 3) in the liver at a dose of 1 mg/l Cd (p<0.05) and on the first day in gill, liver and kidney tissues at doses of 3 and 5 mg/l Cd (p<0.05). The stimulation effect of the 5 mg/l dose of Cd on G6PD and 6PGD enzyme activities was significantly diminished after seven days (Day 7) in all tissues (p<0.05). In contrast to the dose-response pattern at the dose of 5 mg/l Cd, G6PD and 6PGD enzyme activities were stimulated significantly (p<0.05) in liver and kidney tissues at the doses of 3 and 1 mg/l Cd. The stimulation effect of cadmium on the three tissues studied was also calculated; for both of the enzymes (G6PD and 6PGD), the enzyme activity levels were stimulated by approximately 60% and 38% in gills, 68% and 44% in liver, and 67% and 41% in kidneys, respectively, over the base-line enzyme activity of the control groups during the seven-day experimental period. These findings indicate that tissue G6PD and 6PGD enzymes function to protect against cadmium toxicity. |
| Anahtar Kelimeler |
| Enzyme activity | G6PD | Gill | In vivo | Kidney |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Scopus | 15 |