| Yazarlar (4) |
Fatma Cevahir
Türkiye |
|
Önder Düzlü
Erciyes Üniversitesi, Türkiye |
Dr. Öğr. Üyesi Mübeccel ATELGE
Kastamonu Üniversitesi, Türkiye |
|
Alparslan Yıldırım
Erciyes Üniversitesi, Türkiye |
| Özet |
| This study was performed to investigate the phylogenetic characters of the cockroaches in the Kayseri region for mitochondrial cytochrome c oxidase subunit I (mt-COI), mt-COII, and internal transcribed spacer-2 (ITS-2) gene regions. It was also aimed to determine their mechanical transmission of medically important parasites. PCR-restriction fragment length polymorphism (RFLP) was performed by using mt-COI, mt-COII, and ITS-2 DNA gene regions to identify cockroach species (n=220) collected from different regions. Differentiation of cockroach species was based on RFLP models using two restriction enzymes: AvaI and EciI. For phylogenetic analysis, mt-COI, mt-COII, and ITS-2 DNA barcode regions were amplified with standard primers. The obtained amplicons were purified and sequenced using the PCR primers. According to PCR-RFLP, the cockroach species were identified as Blattella germanica (n=105), Blatta orientalis (n=86), and Periplaneta americana (n=29). A total of 13 haplotypes were detected and maximum likelihood (ML) analyses revealed that the sequences of all three species showed a monophyletic structure for the three gene regions. The cockroaches were examined for the presence of parasites. It was found that of the 58 parasitic forms identified, 46 (79.3%) belonged to helminth species and 12 (20.7%) to protozoan species. The results showed that B. germanica (58.6%) had the highest prevalence, followed by Bl. orientalis (32.8%) and P. americana (8.6%). The results of the study not only contribute to the molecular epidemiology of cockroaches but also confirm their important role as mechanical vectors of protozoan and helminth parasites. |
| Anahtar Kelimeler |
| Cockroach | DNA barcoding | ITS-2 | mt-COI and mt-COII | Vector |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale |
| Dergi Adı | ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI |
| Dergi ISSN | 1300-0861 Wos Dergi Scopus Dergi |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q3 |
| Makale Dili | Türkçe |
| Basım Tarihi | 01-2024 |
| Cilt No | 71 |
| Sayı | 4 |
| Sayfalar | 417 / 426 |
| Doi Numarası | 10.33988/auvfd.1300194 |
| Makale Linki | http://vetjournal.ankara.edu.tr/en/pub/issue/86632/1300194 |
| Atıf Sayıları | |
| WoS | 1 |
| Google Scholar | 1 |
