Real time PCR assay for universal detection and quantitation of all five species of fowl adenoviruses FAdV A to FAdV E
Yazarlar (5)
Doç. Dr. Ayşe GÜNEŞ BAYIR Veterinarmedizinische Universitat Wien, Avusturya
Ana Marek
Veterinarmedizinische Universitat Wien, Avusturya
Veterinarmedizinische Universitat Wien, Avusturya
Beatrice Grafl
Veterinarmedizinische Universitat Wien, Avusturya
Veterinarmedizinische Universitat Wien, Avusturya
Mıchael Hess
Veterinarmedizinische Universitat Wien, Avusturya
Veterinarmedizinische Universitat Wien, Avusturya
Evelyn Berger
Veterinarmedizinische Universitat Wien, Avusturya
Veterinarmedizinische Universitat Wien, Avusturya
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Journal of Virological Methods (Q4) | ||
| Dergi ISSN | 0166-0934 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 01-2012 |
| Cilt / Sayı / Sayfa | 183 / 2 / 147–153 | DOI | 10.1016/j.jviromet.2012.04.005 |
| Makale Linki | https://ac.els-cdn.com/S0166093412001322/1-s2.0-S0166093412001322-main.pdf?_tid=0487e23b-e36a-4374-b217-55662a94f8a1acdnat=1551359105_bb94a4b4011042e501ac02bf8f91a50c | ||
| UAK Araştırma Alanları |
Veteriner Gıda Hijyeni ve Teknolojisi
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| Özet |
| The present study describes the development of a SYBR Green based real-time polymerase chain reaction (real-time PCR) for detection and quantitation of all fowl adenovirus (FAdV) species. Primers were designed based on conserved nucleotide sequences within the 52K gene. Ten-fold serial dilutions of a vector DNA were used as standard for quantitation. The real-time PCR had an efficiency of 98%, a regression squared value of 0.999 and showed a range of 6.73-6.73×10(8) copies of FAdV DNA per reaction. The assay was highly specific for FAdVs and an exact quantitation of all 5 FAdV species (FAdV-A to FAdV-E) could be demonstrated. It was shown, that twelve FAdV serotypes (FAdV-1 to 8a, and 8b to 11) were detectable and quantifiable. Other viral genomes as well as uninfected chicken embryo liver (CEL) cells did not produce positive signal. Cloacal swabs were taken during the animal experiment, which was performed with all FAdV species. Shedding of FAdVs was investigated in cell culture, by conventional PCR and by the developed real-time PCR. The real-time PCR was found more sensitive than cell culture and conventional PCR. Detection and quantitation of FAdVs in different type of samples was possible by the new real-time PCR. |
| Anahtar Kelimeler |
| 52K gene | Fowl adenoviruses | Quantitation | Real-time PCR | Sensitivity | Virus shedding |
Science Direct
Real time PCR assay for universal detection and quantitation of all five species of fowl adenoviruses FAdV A to FAdV E
| Atıf Sayıları | |
| Web of Science | 107 |
| Scopus | 116 |
| Google Scholar | 146 |