Effects of Thymol, a Natural Phenolic Compound, on Human Gastric Adenocarcinoma Cells In Vitro.
Yazarlar (4)
Doç. Dr. Ayşe GÜNEŞ BAYIR Bezm-İ Âlem Vakıf Üniversitesi, Türkiye
Prof. Dr. Abdürrahim Koçyiğit Bezm-İ Âlem Vakıf Üniversitesi, Türkiye
Doç. Dr. Eray Metin Güler Bezm-İ Âlem Vakıf Üniversitesi, Türkiye
Hüriye Şenay Kızıltan
Bezm-İ Âlem Vakıf Üniversitesi, Türkiye
Bezm-İ Âlem Vakıf Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Alternative Therapies in Health and Medicine (Q4) | ||
| Dergi ISSN | 1078-6791 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 03-2019 |
| Cilt / Sayı / Sayfa | 24 / 12 / 12–21 | DOI | – |
| Makale Linki | https://www.ncbi.nlm.nih.gov/pubmed/29477139 | ||
| UAK Araştırma Alanları |
Veteriner Gıda Hijyeni ve Teknolojisi
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| Özet |
ContextAlternative and complementary medicine has gained importance in anticancer treatment, reflecting a movement toward an integrated approach to treating various diseases. Natural products originating from plants can contain biologically active substances. Thymol is a major component of many plants from the family Lamiaceae that are often used for medicinal and culinary purposes in Mediterranean countries.ObjectiveThe purpose of the present study was to investigate thymol's cytotoxic, genotoxic, apoptotic effects on gastric adenocarcinoma (AGS) cells, including measurement of reactive oxygen species (ROS) and glutathione (GSH) levels at the same time.DesignThe research team studied thymol's anticancer potential in vitro.SettingThe research was conducted at the Laboratory of Biochemistry of the Faculty of Medicine at Bezmialem Vakif University (Istanbul, Turkey).InterventionCaucasian human AGS cells were exposed to 7 concentrations of thymol-10, 20, 30, 50, 100, 200, and 400 μM-prepared from a stock solution of 600 μM of thymol in dimethyl sulfoxide (DMSO), and after 24 h of incubation, the results were analyzed. The thymol was obtained commercially. The study used a negative control prepared in a concentration of 1:1000 from the stock solution of DMSO.Outcome measuresCytotoxicity was determined using (1) the adenosine 5'-triphosphate cell viability assay; (2) the dichloro-dihydro-fluorescein diacetate assay to evaluate the generation of ROS; (3) the luminescence-based, total GSH assay to determine the GSH levels; and (4) the comet assay to study genotoxicity. Apoptotic induction of thymol was detected (5) by acridine orange/ethidium bromide staining and (6) by Western blotting using a value below the half maximal inhibitory concentration (IC50).ResultsThymol showed significant cytotoxic, genotoxic, apoptotic, ROS-generating, and GSH-reducing effects, in a dose-dependent manner (P ≤ .001). A close negative relationship existed between cell viability and the ROS level.ConclusionsAfter researchers have confirmed thymol's anticarcinogenic effects in vitro on healthy cell lines and in vivo, it may be found to be a novel and strong therapeutic agent against gastric cancer. The study's results suggest that thymol may have therapeutic power when developed from natural components of the diet for treatment of the disease. |
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