| Yazarlar (9) |
Gökhan Zengin
Selçuk Üniversitesi, Türkiye |
|
Abdullahi Ibrahim Uba
Kadir Has Üniversitesi, Türkiye |
Arş. Gör. Mustafa ÖÇAL
Kastamonu Üniversitesi, Türkiye |
|
Majid Sharifi-Rad
University Of Zabol, İran |
|
Giovanni Caprioli
Università Degli Studi Di Camerino, İtalya |
|
Simone Angeloni
Università Degli Studi Di Camerino, İtalya |
Prof. Dr. Yasemin ÇELİK ALTUNOĞLU
Kastamonu Üniversitesi, Türkiye |
Prof. Dr. Mehmet Cengiz BALOĞLU
Kastamonu Üniversitesi, Türkiye |
|
Evren Yıldıztugay
Selçuk Üniversitesi, Türkiye |
| Özet |
| Members of the genus Astragalus have a great interest as a source of natural bioactive compounds on a scientific platform. To provide multidirectional insights into three Astragalus species (A. setulosus, A. anthylloides, and A. ovalis), the current work focused on the chemical characterization and biological properties of their extracts (aerial parts and roots). The chemical characterization of the extracts was detected by HPLC-MS/MS analysis. The biological properties were evaluated by antioxidant, enzyme inhibitory, and cytotoxic parameters. Assays for radical quenching, reducing capacity, and metal chelation were also used to evaluate antioxidant properties. To test the enzyme inhibitory effects of the extracts, cholinesterases, tyrosinase, α-amylase, and α-glucosidase were utilized as target enzymes. Two cancer cell lines, (MCF-7 (human breast cancer cell line) and HeLa (Human cervix cancer cell line), were selected to evaluate cytotoxic effects. Generally, 5- caffeoylquinic acid (2.43–283.92 μg/g extract), hyperoside (4.33–216.22 μg/g extract) and rutin (1.09–184.98 μg/g extract) were the main constituents. The extracts from aerial parts and roots of A. anthylloides showed stronger radical scavenging and reducing power abilities compared to A. setulosus and A. ovalis. The best AChE and BChE inhibitory effects were determined in the aerial parts of A. setulosus (2.18 mg GALAE/g) and roots of A. ovalis (4.76 mg GALAE/g), respectively. The extracts of A. ovalis had the highest tyrosinase inhibitory abilities. The extract from aerial parts of A. setulosus showed stronger cytotoxic effects compared to other extracts. Pearson's correlation analysis revealed that the presence of some compounds (resveratrol, p-coumaric, 5-caffeoylquinic, and ferulic acids, etc) was linked to the observed biological activities. Molecular docking was also provided for the possible interaction of enzymes as well as protein targets of the tested cell lines. Our findings provide a scientific basis for the Astragalus species, which may serve as a source of naturally occurring bioactive compounds for health-promoting applications. |
| Anahtar Kelimeler |
| Astragalus,Phenolics,Cytotoxic effect,Molecular docking,Bioactive agents |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale |
| Dergi Adı | Food Bioscience |
| Dergi ISSN | 2212-4292 Wos Dergi Scopus Dergi |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q1 |
| Makale Dili | Türkçe |
| Basım Tarihi | 01-2022 |
| Cilt No | 49 |
| Sayı | 101858 |
| Sayfalar | 1 / 12 |
| Doi Numarası | 10.1016/j.fbio.2022.101858 |
| Makale Linki | http://dx.doi.org/10.1016/j.fbio.2022.101858 |
| Atıf Sayıları | |
| WoS | 15 |
| SCOPUS | 18 |
| Google Scholar | 22 |
