LC-MS/HRMS Analysis, Anti-Cancer, Anti-Enzymatic and Anti-Oxidant Effects of Boerhavia diffusa Extracts: A Potential Raw Material for Functional Applications
Yazarlar (13)
Kouadio Ibrahime Sinan Selçuk Üniversitesi, Türkiye
Doç. Dr. Uğur AKPULAT Kastamonu Üniversitesi, Türkiye
Afaf A. Aldahish King Khalid University, Suudi Arabistan
Prof. Dr. Yasemin ÇELİK ALTUNOĞLU Kastamonu Üniversitesi, Türkiye
Prof. Dr. Mehmet Cengiz BALOĞLU Kastamonu Üniversitesi, Türkiye
Dimitrina Zheleva-Dimitrova Medical University Of Sofia, Bulgaristan
Reneta Gevrenova Medical University Of Sofia, Bulgaristan
Devina Lobine University Of Mauritius, Mauritius
Mohamad Fawzi Mahomoodally University Of Mauritius, Mauritius
Ouattara Katinan Etienne Université De Cocody-Abidjan
Prof. Dr. Gökhan Zengin Selçuk Üniversitesi, Türkiye
Shafi Mahmud University Of Rajshahi, Bangladeş
Raffaele Capasso Università Degli Studi Di Napoli Federico Iı, İtalya
| Makale Türü |
Özgün Makale
(SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
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| Dergi Adı | Antioxidants (Q2) | ||
| Dergi ISSN | 2076-3921 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 12-2021 |
| Kabul Tarihi | – | Yayınlanma Tarihi | 16-12-2021 |
| Cilt / Sayı / Sayfa | 10 / 12 / 2003–0 | DOI | 10.3390/antiox10122003 |
| Makale Linki | http://dx.doi.org/10.3390/antiox10122003 | ||
| Özet |
| Boerhavia diffusa is a great tropical plant and is widely used for various traditional purposes. In the present study, we examined the influence of solvents (dichloromethane, ethyl acetate, methanol and infusion (water)) on chemical composition and biological capabilities of B. diffusa. An UHPLC-HRMS method was used to determine the chemical characterization. The biological ability was examined for antioxidant, enzyme inhibitory and anti-cancer effects. To evaluate antioxidant effects, different chemical methods (ABTS, DPPH, CUPRAC, FRAP, metal chelating and phosphomolybdenum) were applied. With regard to enzyme inhibitory properties, cholinesterases, amylase, glucosidase and tyrosinase were used. The MDA-MB-231 breast cancer cell line was chosen to determine anticancer activity. Based on the UHPLC-HRMS analysis, 37 specialized metabolites were dereplicated and identified in the studied extracts. Results revealed the presence of 15 hydroxybenzoic, hydroxycinnamic, acylquinic acids, and their glycosides, one rotenoid, seven flavonoids, 12 fatty acids and two other glycosides. Among the tested extracts, the methanol extract showed a stronger antioxidant ability compared with other extracts. The methanol extract also showed the best inhibitory effects on tyrosinase and glucosidase. In the anti-cancer evaluation, the methanol extract showed stronger anticancer effects compared with water extract. In summary, our observations can contribute to the establishment of B. diffusa as a potential candidate for functional applications in the preparation. |
| Anahtar Kelimeler |
| Anti-cancer | Antioxidant | Boerhavia diffusa | Enzyme inhibition | Flavonoids | Functional applications |
