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Antibiotic susceptibility of Acinetobacter spp. isolated from farm animals  
Yazarlar
Doç. Dr. Elçin GÜNAYDIN Doç. Dr. Elçin GÜNAYDIN
Kastamonu Üniversitesi, Türkiye
Gülşen GONCAGÜL
Bursa Uludağ Üniversitesi, Turkey
Pınar MURSALOĞLU KAYNAR
Ankara Medipol University, Turkey
Özlem KARDOĞAN
Kanatlı Hayvan Hastalıkları Teşhis Laboratuvarı, Turkey
Özet
Objective: The genus Acinetobacter is a heterogeneous group of organisms belonging to the Gram-negative coccobacillus group, widely found in nature. These bacteria are isolated from human and animal sources. Antimicrobial agents play a vital role in the treatment of infectious diseases in both humans and animals. Acinetobacter genus antibiotic resistant bacteria are of increasing concern for human and animal health. The aim of this study was to identify bacteria belonging to the genus Acinetobacter from reproductive organs of different animals and their antimicrobial susceptibility. Methods: Our study material consisted of 247 uterine swabs from horses (mare), 56 heifers (cattle), and 32 vaginal swabs from cows in different regions. In bacteriological examination, the samples were incubated in Fluid Thioglycollate Medium (BBL; 221196) at 37 °C for 24 hours. After incubation, isolation studies were carried out on EMB agar (Eosin Methylen-Blue Lactose-Saccharose, BBL; 221355) and Blood Agar (BBL; 297876). Identification of pure cultures obtained as a result of bacterial isolation studies was performed using BBL crystal (Becton-Dickinson, Sparks, USA) Gram positive and Gram negative ID system kits and computer program. The susceptibility of the identified Acinetobacter spp. isolates to different antibiotics was investigated by disc diffusion method. Results: Acinetobacter lwoffii was isolated from 247 uterine swabs from horses and 56 vaginal swabs from heifers (respectively, n = 4 and n=3), while Acinetobacter johnsonii was determined from 32 vaginal swabs from cows (n = 3). Acinetobacter lwoffii was isolated from all samples. Acinetobacter lwoffii which was isolated most frequently in horses. Among the antibiotics tested by disc diffusion method, amikacin, doxycycline and ampisilin/sulbaktama were found to be 100% effective against. In addition to these two antimicrobial agents, 100% susceptibility was detected against netilmicin and gentamicin in heifers where the same agent was isolated. In the antimicrobial susceptibility test applied to Acinetobacter lwoffii isolated in cows, 100% susceptibility was found to ofloxacin in addition to the antimicrobials showing 100% susceptibility in heifers. Conclusion: Acinetobacter spp. may also represent a serious public health problem due to recent evidence of transmission between human and animal. Therefore, we believe that expanding the knowledge about antibiotic-resistant Acinetobacter in the livestock environment will be a resource for future studies.
Anahtar Kelimeler
Acinetobacter | Acinetobacter | antibiotic susceptibility | antibiyotik duyarlılığı | at | cow | düve | heifer | horse | inek
Makale Türü Özgün Makale
Makale Alt Türü SCOPUS dergilerinde yayımlanan tam makale
Dergi Adı Turk Hijyen ve Deneysel Biyoloji Dergisi
Dergi ISSN 0377-9777
Makale Dili İngilizce
Basım Tarihi 01-2024
Cilt No 81
Sayı 4
Sayfalar 379 / 386
Doi Numarası 10.5505/TurkHijyen.2024.86300
BM Sürdürülebilir Kalkınma Amaçları
Atıf Sayıları
Antibiotic susceptibility of Acinetobacter spp. isolated from farm animals

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