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Identification, molecular characterization and expression analysis of RPL24 genes in three Cucurbitaceae family members: cucumber, melon and watermelon    
Yazarlar
Prof. Dr. Mehmet Cengiz BALOĞLU
Kastamonu Üniversitesi, Türkiye
Dr. Öğr. Üyesi Ferhat ULU
Kastamonu Üniversitesi, Türkiye
Prof. Dr. Yasemin ÇELİK ALTUNOĞLU
Kastamonu Üniversitesi, Türkiye
Doç. Dr. Sefa PEKOL
Kastamonu Üniversitesi, Türkiye
Alagöz Gamze
Okan Ese
Özet
Ribosomal proteins are crucial for the proper growth and development of any organism, including plants. The ribosomal protein L24 (RPL24) is found in the large subunit of the ribosome and is responsible for the stabilization of the peptidyl transferase activity. Although RPL24 gene has been individually identified in different organisms, little is known about the genome-wide survey and expression patterns of R24 genes in Cucurbitaceae family members. We identified seven Cucurbitaceae RPL24 genes from cucumber, melon and watermelon. They were phylogenetically clustered into seven major groups. Gene structure and motif composition are relatively conserved in each group. Three-dimensional homology modelling of RPL24 proteins was performed with higher confidence level. CmRPL24-01 was isolated from melon and characterized at a molecular level. The regulation of ribosomal proteins in melon under drought stress conditions was also studied. The expression of CmRPL24-01 gene increased in melon leaf tissue at 3 h upon polyethylene glycol treatment and showed a gradual induction after 12 h. Our study provided a very useful reference for identification and functional analysis of RPL24 protein members in different plants. In addition, this research indicated a potential usage of ribosomal proteins in response to drought stress.
Anahtar Kelimeler
ribosomal protein L24,genome-wide analysis,gene expression analysis,drought stress,Cucurbitaceae family members
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale
Dergi Adı Biotechnology Biotechnological Equipment
Dergi ISSN 1310-2818
Dergi Tarandığı Indeksler SCI-Expanded
Makale Dili İngilizce
Basım Tarihi 09-2015
Cilt No 29
Sayı 6
Sayfalar 1024 / 1034
Doi Numarası 10.1080/13102818.2015.1079144
Makale Linki http://www.tandfonline.com/doi/full/10.1080/13102818.2015.1079144