Free circulating nucleic acids in plasma and serum as a novel approach to the use of internal controls in real time PCR based detection
Yazarlar (5)
Ersin Karatayli Ankara Üniversitesi, Türkiye
Prof. Dr. Yasemin ÇELİK ALTUNOĞLU Kastamonu Üniversitesi, Türkiye
Senem Ceren Karatayli Ankara Üniversitesi, Türkiye
Cihan Yurdaydin Ankara Üniversitesi, Türkiye
A. Mithat Bozdayi Ankara Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Journal of Virological Methods | ||
| Dergi ISSN | 0166-0934 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 07-2014 |
| Kabul Tarihi | – | Yayınlanma Tarihi | 01-10-2014 |
| Cilt / Sayı / Sayfa | 207 / 1 / 133–137 | DOI | 10.1016/j.jviromet.2014.07.008 |
| Makale Linki | http://linkinghub.elsevier.com/retrieve/pii/S016609341400264X | ||
| Özet |
| Internal controls (ICs), are the main components of any real-time PCR based amplification methods, which are co-purified and co-amplified with the actual target. The existence of free circulating nucleic acids in plasma and serum (CNAPS) has been known for many years. The aim of this study was to verify whether CNAPS can be used as ICs in real-time PCR based detection and quantification of DNA or RNA targets in plasma and serum samples. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as a housekeeping gene, was chosen at random as CNAPS to serve as an intrinsic internal control in two different real-time PCR based quantification models in plasma and serum. Viral loads of hepatitis B virus (HBV) DNA and hepatitis delta virus (HDV) RNA were quantified as actual targets in parallel to GAPDH as IC in a total of 519 serum or plasma samples including 21 healthy controls, 202 positive chronic … |
| Anahtar Kelimeler |
| Circulating nucleic acids in serum or plasma | GAPDH | Internal control | Real-time PCR |
Science Direct
Free circulating nucleic acids in plasma and serum as a novel approach to the use of internal controls in real time PCR based detection
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Web of Science | 9 |
| Scopus | 10 |
| Google Scholar | 12 |