A colorimetric immunoassay for the detection of human vascular endothelial growth factor 165 (VEGF165) based on anti-VEGF-iron oxide nanoparticle conjugation
 
Yazarlar (4)
Hülya Kuduğ Ceylan Tokat Gaziosmanpaşa Üniversitesi, Türkiye
Fatma Öztürk Kırbay
Ege Üniversitesi, Türkiye
Prof. Dr. İdris YAZGAN Kastamonu Üniversitesi, Türkiye
Prof. Dr. Murat Elibol Ege Üniversitesi, Türkiye
Makale Türü Açık Erişim Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
Dergi Adı Microchimica Acta (Q1)
Dergi ISSN 0026-3672 Dergi Bilgileri (2024)
Dergi Tarandığı Indeksler SCI-Expanded
Makale Dili Türkçe Basım Tarihi 02-2024
Cilt / Sayı / Sayfa 191 / 3 / 133–0 DOI 10.1007/s00604-024-06228-0
Makale Linki https://doi.org/10.1007/s00604-024-06228-0
UAK Araştırma Alanları
Biyokimya Analitik Kimya
Özet
Vascular endothelial growth factor (VEGF) is an indispensable element in many physiological processes, while alterations in its level in the circulating system are signs of pathology-associated diseases. Therefore, its precise and selective detection is critical for clinical applications to monitor the progression of the pathology. In this study, an optical immunoassay biosensor was developed as a model study for detecting recombinant VEGF165. The VEGF165 sample was purified from recombinant Kluyveromyces lactis GG799 yeast cells. Indirect ELISA was used during the detection, wherein iron oxide nanoparticles (FeNPs) were utilized to obtain optical signals. The FeNPs were synthesized in the presence of lactose p-amino benzoic acid (LpAB). VEGF165 antibody was conjugated to the LpAB-FeNPs through EDC/NHS chemistry to convert the iron oxide nanoparticles into VEGF165 specific probes. The specificity of the prepared system was tested in the presence of potential serum-based interferents (i.e., glucose, urea, insulin, C-reactive protein, and serum amyloid A), and validation studies were performed in a simulated serum sample. The proposed immunoassay showed a wide detection range (0.5 to 100 ng/mL) with a detection limit of 0.29 ng/mL. These results show that the developed assay could offer a sensitive, simple, specific, reliable, and high-throughput detection platform that can be used in the clinical diagnostics of VEGF. Graphical Abstract: (Figure presented.)
Anahtar Kelimeler
Human vascular endothelial growth factor | Immunoassay | Indirect ELISA | Iron oxide nanoparticle | Optical detection
BM Sürdürülebilir Kalkınma Amaçları
Atıf Sayıları
Scopus 13
A colorimetric immunoassay for the detection of human vascular endothelial growth factor 165 (VEGF165) based on anti-VEGF-iron oxide nanoparticle conjugation

Paylaş

İletişim
  • Kastamonu Üniversitesi Rektörlüğü, Kuzeykent Kampüsü, Merkez/Kastamonu
  • unis@kastamonu.edu.tr 03662801000
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