A colorimetric immunoassay for the detection of human vascular endothelial growth factor 165 (VEGF165) based on anti-VEGF-iron oxide nanoparticle conjugation

Ceylan, Hülya Kuduğ; Kırbay, Fatma Öztürk; YAZGAN, İdris; Elibol, Murat

doi:10.1007/s00604-024-06228-0

A colorimetric immunoassay for the detection of human vascular endothelial growth factor 165 (VEGF165) based on anti-VEGF-iron oxide nanoparticle conjugation

Yazarlar (4)

Hülya Kuduğ Ceylan
Tokat Gaziosmanpaşa Üniversitesi, Türkiye

Fatma Öztürk Kırbay
Ege Üniversitesi, Türkiye

Prof. Dr. İdris YAZGAN Kastamonu Üniversitesi, Türkiye

Prof. Dr. Murat Elibol Ege Üniversitesi, Türkiye

Makale Türü	Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
Dergi Adı	Microchimica Acta (Q1)
Dergi ISSN	0026-3672 Wos Dergi Scopus Dergi
Dergi Tarandığı Indeksler	SCI-Expanded
Makale Dili	Türkçe	Basım Tarihi	02-2024
Cilt / Sayı / Sayfa	191 / 3 / 133–0	DOI	10.1007/s00604-024-06228-0
Makale Linki	https://doi.org/10.1007/s00604-024-06228-0

Özet

Vascular endothelial growth factor (VEGF) is an indispensable element in many physiological processes, while alterations in its level in the circulating system are signs of pathology-associated diseases. Therefore, its precise and selective detection is critical for clinical applications to monitor the progression of the pathology. In this study, an optical immunoassay biosensor was developed as a model study for detecting recombinant VEGF165. The VEGF165 sample was purified from recombinant Kluyveromyces lactis GG799 yeast cells. Indirect ELISA was used during the detection, wherein iron oxide nanoparticles (FeNPs) were utilized to obtain optical signals. The FeNPs were synthesized in the presence of lactose p-amino benzoic acid (LpAB). VEGF165 antibody was conjugated to the LpAB-FeNPs through EDC/NHS chemistry to convert the iron oxide nanoparticles into VEGF165 specific probes. The specificity of the prepared system was tested in the presence of potential serum-based interferents (i.e., glucose, urea, insulin, C-reactive protein, and serum amyloid A), and validation studies were performed in a simulated serum sample. The proposed immunoassay showed a wide detection range (0.5 to 100 ng/mL) with a detection limit of 0.29 ng/mL. These results show that the developed assay could offer a sensitive, simple, specific, reliable, and high-throughput detection platform that can be used in the clinical diagnostics of VEGF. Graphical Abstract: (Figure presented.)

Anahtar Kelimeler

Human vascular endothelial growth factor | Immunoassay | Indirect ELISA | Iron oxide nanoparticle | Optical detection

BM Sürdürülebilir Kalkınma Amaçları

Atıf Sayıları
Scopus	8

A colorimetric immunoassay for the detection of human vascular endothelial growth factor 165 (VEGF165) based on anti-VEGF-iron oxide nanoparticle conjugation

Dergi Adı	MICROCHIMICA ACTA
Yayıncı	Springer-Verlag Wien
Açık Erişim	Hayır
ISSN	0026-3672
E-ISSN	1436-5073
CiteScore	7,8
SJR	0,833
SNIP	0,804

A colorimetric immunoassay for the detection of human vascular endothelial growth factor 165 (VEGF165) based on anti-VEGF-iron oxide nanoparticle conjugation

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