A one step real time PCR method for the quantification of hepatitis delta virus RNA using an external armored RNA standard and intrinsic internal control
Yazarlar (9)
Ersin Karatayli Ankara Üniversitesi, Türkiye
Prof. Dr. Yasemin ÇELİK ALTUNOĞLU Kastamonu Üniversitesi, Türkiye
Senem Ceren Karatayli Ankara Üniversitesi, Türkiye
S. Gökçe K. Alagöz Ankara Üniversitesi, Türkiye
Kubilay Çinar Ankara Üniversitesi, Türkiye
Prof. Dr. Kendal Yalçin Dicle Üniversitesi, Türkiye
Ramazan Idilman Ankara Üniversitesi, Türkiye
Cihan Yurdaydın Ankara Üniversitesi, Türkiye
A. Mithat Bozdayi Ankara Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Journal of Clinical Virology | ||
| Dergi ISSN | 1386-6532 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 02-2014 |
| Kabul Tarihi | – | Yayınlanma Tarihi | 01-05-2014 |
| Cilt / Sayı / Sayfa | 60 / 1 / 11–15 | DOI | 10.1016/j.jcv.2014.01.021 |
| Makale Linki | http://linkinghub.elsevier.com/retrieve/pii/S1386653214000419 | ||
| Özet |
| BackgroundHepatitis delta virus (HDV) RNA viral load measurement is critical in diagnosis and monitoring the response to antiviral treatment.ObjectivesOur aim is to design a real time PCR method for accurate quantitation of HDV RNA in clinical specimens using an armored RNA as external standard, and an intrinsic internal control.Study designA plasmid bearing delta antigen region of genotype I HDV genome was used to develop an armored RNA. Serial dilutions of the armored HDV RNA standard with 1012 copy/mL were used as standards for quantitation. A primer–probe set derived from HDAg region was used in one step EZ RT PCR kit chemistry which uses rTth enzyme allowing reverse transcription and polymerization in the same tube. The kit also uses the advantage of uracil-N-glycosylase (UNG) enzyme treatment to prevent PCR contamination.ResultsThe established assay has a dynamic range of 102 … |
| Anahtar Kelimeler |
| Armored RNA | HDV | Intrinsic control | Real time PCR |
Science Direct
A one step real time PCR method for the quantification of hepatitis delta virus RNA using an external armored RNA standard and intrinsic internal control
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Web of Science | 26 |
| Scopus | 31 |
| Google Scholar | 47 |