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Exploring the Molecular Basis of Anticancer Activity in Various Mushroom Species Against Colorectal Cancer Cells     
Yazarlar (5)
İpek Ceylan
Doç. Dr. Dilşad ÖZERKAN Doç. Dr. Dilşad ÖZERKAN
Kastamonu Üniversitesi, Türkiye
Doç. Dr. Nuray EMİN Doç. Dr. Nuray EMİN
Kastamonu Üniversitesi, Türkiye
Ilgaz Akata
Ankara Üniversitesi, Türkiye
Prof. Dr. Ergin Murat ALTUNER Prof. Dr. Ergin Murat ALTUNER
Kastamonu Üniversitesi, Türkiye
Devamını Göster
Özet
Background: Mushrooms are shown to protect against the side effects of cancer. Therefore, mushrooms with proven anticancer properties and active ingredients are fascinating in the search for new cancer drugs. Objective: In this study, the effects of extracts from Hericium coralloides (M1), Lactarius deliciosus (M2), Lepista nuda (M3), Pleurotus ostreatus (M4), and Suillus collitinus (M5) together on HCT116 were investigated. Mesenchymal stem cells (MSCs) were used to study the effect on healthy cells. Methods: MTT was used to determine cell viability. Dose-response curves were generated, the IC50 values of the compounds were calculated, and the effect of the extracts was compared using it. The FTIR was used to analyze the quantitative changes of the cellular components. Results and Discussion: The evaluation of the IC50 values of all fungal species showed that they reduced the cell viability of HCT116 cells. In contrast, no significant reduction in cell viability was observed in MSCs. Changes in the ratio of cell membrane lipids, proteins, and cell nucleic acids between control and fungal-treated HCT116 cells were detected by FTIR. Many of the chemotherapeutic agents are of plant origin, and many resources should still be explored to inhibit the side effects of cancer therapy. Conclusion: The data obtained through this experiment will serve as a reference for studies to be a new source of anticancer drugs in modern pharmacology.
Anahtar Kelimeler
Antiproliferation | colorectal cancer | cytotoxicity | FTIR spectroscopy | mosses | research and development (R&D)
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale
Dergi Adı Letters in Drug Design & Discovery
Dergi ISSN 1570-1808
Dergi Tarandığı Indeksler SCI
Dergi Grubu Q4
Makale Dili İngilizce
Basım Tarihi 01-2024
Sayı 1
Doi Numarası 10.2174/0115701808324049241108063414