The extent of multiallelic, co‐editing of LIGULELESS1 in highly polyploid sugarcane tunes leaf inclination angle and enables selection of the ideotype for biomass yield

Brant, Eleanor; Eid, Ayman; Kannan, Baskaran; BALOĞLU, Mehmet Cengiz; Altpeter, Fredy

doi:10.1111/pbi.14380

The extent of multiallelic, co‐editing of LIGULELESS1 in highly polyploid sugarcane tunes leaf inclination angle and enables selection of the ideotype for biomass yield

Yazarlar (5)

Eleanor Brant
University Of Florida Institute Of Food And Agricultural Sciences, Amerika Birleşik Devletleri

Ayman Eid
University Of Florida Institute Of Food And Agricultural Sciences, Amerika Birleşik Devletleri

Baskaran Kannan
University Of Florida Institute Of Food And Agricultural Sciences, Amerika Birleşik Devletleri

Prof. Dr. Mehmet Cengiz BALOĞLU Kastamonu Üniversitesi, Türkiye

Fredy Altpeter
University Of Florida Institute Of Food And Agricultural Sciences, Amerika Birleşik Devletleri

Makale Türü	Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
Dergi Adı	Plant Biotechnology Journal (Q1)
Dergi ISSN	1467-7644 Wos Dergi Scopus Dergi
Dergi Tarandığı Indeksler	SCI
Makale Dili	Türkçe	Basım Tarihi	05-2024
Cilt / Sayı / Sayfa	22 / 10 / 2660–2671	DOI	10.1111/pbi.14380
Makale Linki	https://doi.org/10.1111/pbi.14380
UAK Araştırma Alanları	Biyoteknoloji

Özet

SummarySugarcane (Saccharum spp. hybrid) is a prime feedstock for commercial production of biofuel and table sugar. Optimizing canopy architecture for improved light capture has great potential for elevating biomass yield. LIGULELESS1 (LG1) is involved in leaf ligule and auricle development in grasses. Here, we report CRISPR/Cas9‐mediated co‐mutagenesis of up to 40 copies/alleles of the putative LG1 in highly polyploid sugarcane (2n = 100–120, x = 10–12). Next generation sequencing revealed co‐editing frequencies of 7.4%–100% of the LG1 reads in 16 of the 78 transgenic lines. LG1 mutations resulted in a tuneable leaf angle phenotype that became more upright as co‐editing frequency increased. Three lines with loss of function frequencies of ~12%, ~53% and ~95% of lg1 were selected following a randomized greenhouse trial and grown in replicated, multi‐row field plots. The co‐edited LG1 mutations were stably maintained in vegetative progenies and the extent of co‐editing remained constant in field tested lines L26 and L35. Next generation sequencing confirmed the absence of potential off targets. The leaf inclination angle corresponded to light transmission into the canopy and tiller number. Line L35 displaying loss of function in ~12% of the lg1 NGS reads exhibited an 18% increase in dry biomass yield supported by a 56% decrease in leaf inclination angle, a 31% increase in tiller number, and a 25% increase in internode number. The scalable co‐editing of LG1 in highly polyploid sugarcane allows fine‐tuning of leaf inclination angle, enabling the selection of the ideotype for biomass yield.

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Atıf Sayıları
Scopus	27
Google Scholar	32

The extent of multiallelic, co‐editing of LIGULELESS1 in highly polyploid sugarcane tunes leaf inclination angle and enables selection of the ideotype for biomass yield

Dergi Adı	PLANT BIOTECHNOLOGY JOURNAL
Yayıncı	Wiley-Blackwell Publishing Ltd
Açık Erişim	Evet
ISSN	1467-7644
E-ISSN	1467-7652
CiteScore	17,5
SJR	2,907
SNIP	2,183

The extent of multiallelic, co‐editing of LIGULELESS1 in highly polyploid sugarcane tunes leaf inclination angle and enables selection of the ideotype for biomass yield

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