| Yazarlar (7) |
Semra Tasdurmazli
Yıldız Teknik Üniversitesi, Turkey |
Doç. Dr. İrfan ÇINAR
Kastamonu Üniversitesi, Türkiye |
|
Murat Karamese
Kafkas Üniversitesi, Turkey |
|
Selina Aksak Karamese
Kafkas Üniversitesi, Turkey |
|
Elif Cadirci
Ataturk University, Faculty of Medicine, Turkey |
|
Luís D.R. Melo
Universidade do Minho, Portugal |
|
Tulin Ozbek
Yıldız Teknik Üniversitesi, Turkey |
| Özet |
| The increasing threat of antimicrobial-resistant bacteria, particularly Staphylococcus aureus, which rapidly develops multidrug resistance and commonly colonizes wound surfaces, demands innovative strategies. Phage-encoded endolysins offer a dual-purpose approach as topical therapies for infectious skin wounds and synergistic agents to reduce high-dose antibiotic dependence. This study explores recombinant CHAPk (rCHAPk), efficiently synthesized within 3 h, displaying broad-spectrum antibacterial activity against 10 Gram-positive strains, including resistant variants, with rapid bactericidal kinetics. Application of 10 μg of rCHAPk reduced OD600 by 0.4 within 5 min against a clinical methicillin-resistant S. aureus (MRSA) strain. Combining rCHAPk (1.875 μg/mL) with oxacillin/vancomycin lowered their minimum bactericidal concentrations to 1 μg/mL from initial values over 64 μg/mL and 32 μg/mL, respectively, with a fractional inhibitory concentration index below 0.1. rCHAPk retained efficacy after one year of refrigerated storage. In in vivo experiments, rCHAPk outperformed commercial fucidin therapy in MRSA-induced murine wound models over two weeks, enhancing wound healing by modulating pro-inflammatory cytokine responses and the proliferative phase. This study, for the first time, investigates rCHAPk's in vitro combination with antibiotics and wound healing parameters, highlighting its potential as a potent antibacterial agent synergizing with antibiotics to address antibiotic-resistant bacterial wound infections. |
| Anahtar Kelimeler |
| Endolysin rCHAPk | Endolysin-antibiotic synergism | MRSA-infected in vivo wound model |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | International Journal of Biological Macromolecules |
| Dergi ISSN | 0141-8130 |
| Dergi Grubu | Q1 |
| Makale Dili | İngilizce |
| Basım Tarihi | 03-2025 |
| Cilt No | 296 |
| Sayı | 1 |
| Doi Numarası | 10.1016/j.ijbiomac.2025.139630 |
| Atıf Sayıları |
