| Yazarlar (13) |
Tülin Özbek
Türkiye |
|
Hatice Demir
|
|
Senanur Dokuz
|
|
Semra Taşdurmazlı
|
|
Utku Özbey
|
|
Mehmet Özbil
Türkiye |
|
Murat Topuzoğulları
Türkiye |
Doç. Dr. İrfan ÇINAR
Kastamonu Üniversitesi, Türkiye |
|
Murat Karameşe
Türkiye |
|
Selina Aksak Karameşe
Türkiye |
|
Serap Acar
Türkiye |
|
Ömer Faruk Bayrak
Türkiye |
|
Elif Çadırcı
Türkiye |
| Özet |
| The rapid increase in bacterial resistance to existing treatments underscores the critical need for novel therapeutic strategies. Here, an innovative approach using targeted nanocarrier systems that mimic phage-bacteria interactions through phage receptor binding protein (Gp45 from the ϕ11 lysogenic phage) or derived peptides (P1, P2, P3, P4, P5), are introduced. These nanodrugs, exhibited receptor-ligand specificity and strong binding affinity, for the first time, were employed for the precise delivery and targeting of antibiotics within living organisms. The actively targeted micelles via two methods were produced; conjugating GP45 to dual antibiotic-loaded PLGA-b-PEG micelles (MiGp45) and the synthesis of peptide-conjugated micelles with dual antibiotic-loaded PLGA-b-PEG-peptide triblock copolymers. The untargeted nano-drug reduced MIC values by 2–10 times for vancomycin and 9–75 times for oxacillin, resulting in a synergistic effect. MiGp45 and MiP1-targeted micelles further reduced MIC values at least twofold, up to ninefold in resistant strains, indicating significant antibacterial improvement. In a mouse model of sepsis by S. aureus, MiGp45 treatment resulted in complete recovery as opposed to death in the untreated group, significantly reduced bacterial load, pro-inflammatory cytokine expression, lung injury, and normalized oxidative stress. The phage-based nanodrugs show tremendous promise as a highly effective antimicrobial treatment targeting multidrug- resistant pathogens. |
| Anahtar Kelimeler |
| Dual antibiotic-loaded nanodrug | Phage-based targeting | Pneumonia sepsis | S. aureus infection |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | JOURNAL OF CONTROLLED RELEASE |
| Dergi ISSN | 0168-3659 Wos Dergi Scopus Dergi |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Dergi Grubu | Q1 |
| Makale Dili | Türkçe |
| Basım Tarihi | 01-2025 |
| Cilt No | 380 |
| Sayı | 1 |
| Sayfalar | 14 / 786 |
| Doi Numarası | 10.1016/j.jconrel.2025.02.035 |
| Atıf Sayıları |
