| Yazarlar (6) |
Muhammed Yayla
Selçuk Tip Fakültesi, Turkey |
|
Erdem Toktay
Kafkas Üniversitesi, Türkiye |
|
Bengul Ozdemir Sarikaya
Kafkas Üniversitesi, Türkiye |
|
Ugur Ermis
Kafkas Üniversitesi, Türkiye |
|
Sakir Akgun
Kafkas Üniversitesi, Türkiye |
Doç. Dr. İrfan ÇINAR
Kastamonu Üniversitesi, Türkiye |
| Özet |
| Purpose: The physiological effects of 5-HT7 receptors expressed in pancreatic beta cells have not yet been elucidated. We first aimed to investigate the effect of 5-HT7 receptor agonist (AS19) and antagonist (SB269970) application on insulin secretion in RIN-5F pancreatic beta cells. Subsequently, we aimed to investigate the effects of agonist and antagonist applications on cell damage induced by STZ. Materials/Methods: Cell damage was caused by giving 5 mM STZ solution to the cells for 12 h. The protective effects of 5-HT7 receptor agonist and antagonist on this subsequent damage were investigated. IGF-1, TNF-α, TGF-B1, NF-KB, Bax, Caspase 3, Caspase 9, 5-HT7, 5HT7x2, 5HT7x3 mRNA expression levels were compared between groups. Results: While agonist application stimulates insulin secretion, the effect of the antagonist varies.SB269970 reduced oxidative stress and downregulated TNF-a, TGF-B1 and NF-KB expression and also prevented apoptosis by decreasing Bax, caspase 3 and 9 levels against STZ-induced beta cell damage. Conclusions: The effect of 5-HT7 receptors on insulin secretion and their effects against STZ damage will be guiding for more detailed studies in the treatment of diabetes and related diseases in the future. |
| Anahtar Kelimeler |
| 5-HT7 | AS19 | Insulin | RIN-5F | SB269970 |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | Toxicology in Vitro |
| Dergi ISSN | 0887-2333 Wos Dergi Scopus Dergi |
| Dergi Grubu | Q3 |
| Makale Dili | İngilizce |
| Basım Tarihi | 06-2025 |
| Cilt No | 106 |
| Sayı | 1 |
| Doi Numarası | 10.1016/j.tiv.2025.106050 |
| Atıf Sayıları |
