Comparative transcriptomic analysis provides novel insights on the hormonal regulation of postharvest apples under cold storage
Yazarlar (9)
Prof. Dr. Ufuk Çelikkol Akçay Isparta University Of Applied Sciences, Türkiye
Prof. Dr. Mehmet Cengiz BALOĞLU Kastamonu Üniversitesi, Türkiye
Prof. Dr. Yasemin ÇELİK ALTUNOĞLU Kastamonu Üniversitesi, Türkiye
Pınar Baloğlu
Kastamonu Üniversitesi, Türkiye
Prof. Dr. Mehmet Ali Koyuncu Isparta University Of Applied Sciences, Türkiye
Doç. Dr. Derya Erbaş Isparta University Of Applied Sciences, Türkiye
Erdogan Horuz
Kastamonu Üniversitesi, Türkiye
Dr. Öğr. Üyesi Büşra Arslan Kastamonu Üniversitesi, Türkiye
Sule Turkoglu
Isparta University Of Applied Sciences, Türkiye
Makale Türü Açık Erişim Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
Dergi Adı Horticulture Environment and Biotechnology (Q1)
Dergi ISSN 2211-3452 Dergi Bilgileri (2025)
Dergi Tarandığı Indeksler SCI-Expanded
Makale Dili Türkçe Basım Tarihi 03-2025
Cilt / Sayı / Sayfa 66 / 5 / 1089–1105 DOI 10.1007/s13580-025-00710-1
Makale Linki https://doi.org/10.1007/s13580-025-00710-1
UAK Araştırma Alanları
Biyoteknoloji
Özet
Abstract
Postharvest storage is a crucial phase during which fruit undergoes ripening and senescence. This study is based on the hypothesis that ethylene-auxin interactions play a significant role in determining apple storage life and quality during the postharvest period. To test this hypothesis, fruit flesh firmness and hormone levels were analyzed alongside transcriptomic changes observed through RNA sequencing. A comprehensive investigation of hormone metabolism-related gene expression across the whole transcriptome was conducted in ‘Golden Delicious’ apples over a six-month storage period to elucidate the molecular mechanisms underlying postharvest ripening and senescence. Understanding changes in the ripening process was facilitated by pretreatment with ethylene, the ethylene inhibitor 1-methylcyclopropene (1-MCP), auxin, and the auxin inhibitor 1-N-naphthylphthalamic acid (NPA) prior to cold storage. The results demonstrated that the auxin inhibitor suppressed ethylene production along with auxin levels, while the ethylene inhibitor suppressed auxin levels together with ethylene production throughout all storage periods. Findings also revealed that ethylene and auxin hormones regulate key metabolic processes associated with apple ripening during storage. Additionally, many transcriptional regulatory genes involved in hormone signaling and metabolism, such as AIL5, ABI3, AP2, ERF-011, NAC, RAP210, and REVEILLE, exhibited high activity during storage. Furthermore, this study revealed the high expression levels of ABC transporter and LRR receptor protein kinase for the first time in apples under storage conditions. These findings provide deeper insights into the physiological and molecular changes occurring in climacteric fruit during postharvest storage.
Anahtar Kelimeler
1-MCP | Auxin | Ethylene | NPA | Transcriptomics
BM Sürdürülebilir Kalkınma Amaçları
Atıf Sayıları
Scopus 4
Google Scholar 4
Comparative transcriptomic analysis provides novel insights on the hormonal regulation of postharvest apples under cold storage

Paylaş

İletişim
  • Kastamonu Üniversitesi Rektörlüğü, Kuzeykent Kampüsü, Merkez/Kastamonu
  • unis@kastamonu.edu.tr 03662801000
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