Comparative transcriptomic analysis provides novel insights on the hormonal regulation of postharvest apples under cold storage

Akçay, Ufuk Çelikkol; BALOĞLU, Mehmet Cengiz; ÇELİK ALTUNOĞLU, Yasemin; Baloğlu, Pınar; Koyuncu, Mehmet Ali; Erbaş, Derya; Horuz, Erdogan; Arslan, Büşra; Turkoglu, Sule

doi:10.1007/s13580-025-00710-1

Comparative transcriptomic analysis provides novel insights on the hormonal regulation of postharvest apples under cold storage

Yazarlar (9)

Prof. Dr. Ufuk Çelikkol Akçay Isparta University Of Applied Sciences, Türkiye

Prof. Dr. Mehmet Cengiz BALOĞLU Kastamonu Üniversitesi, Türkiye

Prof. Dr. Yasemin ÇELİK ALTUNOĞLU Kastamonu Üniversitesi, Türkiye

Pınar Baloğlu
Kastamonu Üniversitesi, Türkiye

Prof. Dr. Mehmet Ali Koyuncu Isparta University Of Applied Sciences, Türkiye

Doç. Dr. Derya Erbaş Isparta University Of Applied Sciences, Türkiye

Erdogan Horuz
Kastamonu Üniversitesi, Türkiye

Dr. Öğr. Üyesi Büşra Arslan Kastamonu Üniversitesi, Türkiye

Sule Turkoglu
Isparta University Of Applied Sciences, Türkiye

Makale Türü	Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
Dergi Adı	Horticulture Environment and Biotechnology (Q2)
Dergi ISSN	2211-3452 Wos Dergi Scopus Dergi
Dergi Tarandığı Indeksler	SCI-Expanded
Makale Dili	Türkçe	Basım Tarihi	03-2025
Cilt / Sayı / Sayfa	66 / 5 / 1089–1105	DOI	10.1007/s13580-025-00710-1
Makale Linki	https://doi.org/10.1007/s13580-025-00710-1
UAK Araştırma Alanları	Biyoteknoloji

Özet

Abstract
Postharvest storage is a crucial phase during which fruit undergoes ripening and senescence. This study is based on the hypothesis that ethylene-auxin interactions play a significant role in determining apple storage life and quality during the postharvest period. To test this hypothesis, fruit flesh firmness and hormone levels were analyzed alongside transcriptomic changes observed through RNA sequencing. A comprehensive investigation of hormone metabolism-related gene expression across the whole transcriptome was conducted in ‘Golden Delicious’ apples over a six-month storage period to elucidate the molecular mechanisms underlying postharvest ripening and senescence. Understanding changes in the ripening process was facilitated by pretreatment with ethylene, the ethylene inhibitor 1-methylcyclopropene (1-MCP), auxin, and the auxin inhibitor 1-N-naphthylphthalamic acid (NPA) prior to cold storage. The results demonstrated that the auxin inhibitor suppressed ethylene production along with auxin levels, while the ethylene inhibitor suppressed auxin levels together with ethylene production throughout all storage periods. Findings also revealed that ethylene and auxin hormones regulate key metabolic processes associated with apple ripening during storage. Additionally, many transcriptional regulatory genes involved in hormone signaling and metabolism, such as AIL5, ABI3, AP2, ERF-011, NAC, RAP210, and REVEILLE, exhibited high activity during storage. Furthermore, this study revealed the high expression levels of ABC transporter and LRR receptor protein kinase for the first time in apples under storage conditions. These findings provide deeper insights into the physiological and molecular changes occurring in climacteric fruit during postharvest storage.

Anahtar Kelimeler

1-MCP | Auxin | Ethylene | NPA | Transcriptomics

BM Sürdürülebilir Kalkınma Amaçları

Atıf Sayıları
Scopus	3
Google Scholar	3

Comparative transcriptomic analysis provides novel insights on the hormonal regulation of postharvest apples under cold storage

Dergi Adı	Horticulture Environment and Biotechnology
Yayıncı	Springer
Açık Erişim	Hayır
ISSN	2211-3452
E-ISSN	2211-3460
CiteScore	4,7
SJR	0,522
SNIP	0,916

Comparative transcriptomic analysis provides novel insights on the hormonal regulation of postharvest apples under cold storage

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