Development of a sugar-derived silver nanoparticle-based electrochemical immunosensor for sensitive D-dimer detection
Yazarlar (3)
Fatma Öztürk Kırbay
Ege Üniversitesi, Türkiye
Ege Üniversitesi, Türkiye
Prof. Dr. İdris YAZGAN Kastamonu Üniversitesi, Türkiye
Prof. Dr. Dilek Odacı Demirkol Ege Üniversitesi, Türkiye
| Makale Türü |
Özgün Makale
(SCOPUS dergilerinde yayınlanan tam makale)
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| Dergi Adı | Sensors International | ||
| Dergi ISSN | 2666-3511 Scopus Dergi | ||
| Dergi Tarandığı Indeksler | esci | ||
| Makale Dili | İngilizce | Basım Tarihi | 03-2025 |
| Cilt / Sayı / Sayfa | 6 / 1 / 100332–0 | DOI | 10.1016/j.sintl.2025.100332 |
| Makale Linki | https://doi.org/10.1016/j.sintl.2025.100332 | ||
| UAK Araştırma Alanları |
Biyokimya
Analitik Kimya
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| Özet |
| D-dimer (DD) is a key marker of coagulation and fibrinolysis activation. Clinicians can make important decisions with the aid of quick analysis and quantification, ideally at the patient's bedside. Electrochemical immunosensors are effective point-of-care technologies to address these issues. Here, we describe the development of a simple electrochemical immunosensor to detect DD. The immunosensor is constructed by electrodeposition of lactose methoxide aniline silver nanoparticles (LMA-AgNPs) on a screen-printed carbon electrode (SPCE). The LMA-AgNP and SPCE/LMA-AgNP were characterized by Fourier transform infrared (FTIR) spectroscopy, high-resolution transmission electron microscopy (HR-TEM), scanning electron microscopy-energy dispersive X-ray spectroscopy (SEM-EDS), and X-ray photoelectron spectroscopy (XPS). The electrochemical behavior of the SPCE/LMA-AgNP was examined using electrochemical impedance spectroscopy (EIS), differential pulse voltammetry (DPV), and cyclic voltammetry (CV). Then, Anti-D-Dimer antibody (Anti-DD) was immobilized on SPCE/LMA-AgNP. Along with the analytical characterization of the SPCE/LMA-AgNP/Anti-DD immunosensor, the linear range for DD, limit of detection (LOD), potential chemical interferences, and real sample applications were also evaluated. The antigen/antibody interaction at various DD concentrations was monitored using DPV. The SPCE/LMA-AgNP/Anti-DD shows a linear response in the DD concentration range of 0.01–1 pg/mL and a LOD of 0.2 fg/mL. In addition, the SPCE/LMA-AgNP/Anti-DD showed excellent specificity and no response to potential interfering substances, for instance urea, insulin, C-reactive protein (CRP), and serum amyloid A (SAA). Eventually, the SPCE/LMA-AgNP/Anti-DD is applied in human serum, which shows good recovery values (97.2 %). The SPCE/LMA-AgNP/Anti-DD can be fabricated cheaply and easily for bedside clinical evaluation. |
| Anahtar Kelimeler |
| Biomarker | D-dimer | Electrodeposition | Immunosensor | Silver nanoparticle |
Science Direct
Development of a sugar-derived silver nanoparticle-based electrochemical immunosensor for sensitive D-dimer detection
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Scopus | 1 |