| Yazarlar (3) |
Fatma Ozturk Kirbay
Ege Üniversitesi, Türkiye |
Doç. Dr. İdris YAZGAN
Kastamonu Üniversitesi, Türkiye |
|
Dilek Odaci
Ege Üniversitesi, Türkiye |
| Özet |
| D-dimer (DD) is a key marker of coagulation and fibrinolysis activation. Clinicians can make important decisions with the aid of quick analysis and quantification, ideally at the patient's bedside. Electrochemical immunosensors are effective point-of-care technologies to address these issues. Here, we describe the development of a simple electrochemical immunosensor to detect DD. The immunosensor is constructed by electrodeposition of lactose methoxide aniline silver nanoparticles (LMA-AgNPs) on a screen-printed carbon electrode (SPCE). The LMA-AgNP and SPCE/LMA-AgNP were characterized by Fourier transform infrared (FTIR) spectroscopy, high-resolution transmission electron microscopy (HR-TEM), scanning electron microscopy-energy dispersive X-ray spectroscopy (SEM-EDS), and X-ray photoelectron spectroscopy (XPS). The electrochemical behavior of the SPCE/LMA-AgNP was examined using electrochemical impedance spectroscopy (EIS), differential pulse voltammetry (DPV), and cyclic voltammetry (CV). Then, Anti-D-Dimer antibody (Anti-DD) was immobilized on SPCE/LMA-AgNP. Along with the analytical characterization of the SPCE/LMA-AgNP/Anti-DD immunosensor, the linear range for DD, limit of detection (LOD), potential chemical interferences, and real sample applications were also evaluated. The antigen/antibody interaction at various DD concentrations was monitored using DPV. The SPCE/LMA-AgNP/Anti-DD shows a linear response in the DD concentration range of 0.01–1 pg/mL and a LOD of 0.2 fg/mL. In addition, the SPCE/LMA-AgNP/Anti-DD showed excellent specificity and no response to potential interfering substances, for instance urea, insulin, C-reactive protein (CRP), and serum amyloid A (SAA). Eventually, the SPCE/LMA-AgNP/Anti-DD is applied in human serum, which shows good recovery values (97.2 %). The SPCE/LMA-AgNP/Anti-DD can be fabricated cheaply and easily for bedside clinical evaluation. |
| Anahtar Kelimeler |
| Biomarker | D-dimer | Electrodeposition | Immunosensor | Silver nanoparticle |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SCOPUS dergilerinde yayımlanan tam makale |
| Dergi Adı | Sensors International |
| Dergi ISSN | 2666-3511 |
| Makale Dili | İngilizce |
| Basım Tarihi | 01-2025 |
| Cilt No | 6 |
| Sayı | 1 |
| Doi Numarası | 10.1016/j.sintl.2025.100332 |
| Atıf Sayıları |
