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Akademisyenler > Aysun İNAN GENÇ > Yayın Detayı

Therapeutic potential of young plasma in reversing age-related liver inflammation via modulation of NLRP3 inflammasome and necroptosis     
Yazarlar (7)
Burcu Baba
Yüksek İhtisas Üniversitesi, Türkiye
Taha Ceylani
Muş Alparslan Üniversitesi, Türkiye
Hikmet Taner Teker
Ankara Medipol Üniversitesi, Türkiye
Seda Keskin
Van Yüzüncü Yıl Üniversitesi, Türkiye
Arş. Gör. Dr. Aysun İNAN GENÇ Arş. Gör. Dr. Aysun İNAN GENÇ
Kastamonu Üniversitesi, Türkiye
Rafig Gurbanov
Bilecik Şeyh Edebali Üniversitesi, Türkiye
Eda Açıkgöz
Van Yüzüncü Yıl Üniversitesi, Türkiye
Devamını Göster
Özet
The phenomenon of inflammaging, characterized by an increase in low-grade chronic inflammation, is closely associated with diseases related to liver dysfunction. This study investigated daily plasma exchange between 5-week-old and 24-month-old Sprague Dawley rats for 30 days, focusing on protein secondary structures, NLRP3 inflammasome, and necroptosis. Conformation changes in protein secondary structures were identified by infrared spectroscopy-based pattern recognition analysis. Liver biopsies with histochemical and immunohistochemical staining were used to assess molecules associated with inflammation, necroptosis and NLRP3 inflammasome complex. Expression levels of NLRP3 components were determined by qPCR. Enhanced random coils, 310 helices, β-turns, and loop structures were identified in old rats and young rats with old plasma. Young rats and old rats with young plasma displayed higher α-helices and β-sheet structures. Young rats with old plasma showed increased NLRP3, ASC, caspase-1, IL-1β, and IL-18 mRNA levels, indicating an inflammatory response. Whereas old rats with young plasma exhibited lower inflammation levels. Histological evaluations revealed that young rats receiving aged plasma showed significantly increased levels of NLRP3, ASC, caspase-1, IL-1β, TNF-α, VEGFR2, RIPK1, and MLKL immunoreactivity, whereas decreased immunoreactivity in aged rats receiving young plasma. These findings suggest that young plasma reduces NLRP3 inflammasome activation and necroptosis in aged rats.
Anahtar Kelimeler
Inflammaging | Infrared spectroscopy | Necroptosis | NLRP3 Inflammasome | Plasma exchange
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale
Dergi Adı Biogerontology
Dergi ISSN 1389-5729 Wos Dergi Scopus Dergi
Dergi Tarandığı Indeksler
Dergi Grubu Q2
Makale Dili İngilizce
Basım Tarihi 05-2025
Cilt No 26
Sayı 117
Doi Numarası 10.1007/s10522-025-10260-9
Makale Linki https://link.springer.com/article/10.1007/s10522-025-10260-9
BM Sürdürülebilir Kalkınma Amaçları
Atıf Sayıları
Therapeutic potential of young plasma in reversing age-related liver inflammation via modulation of NLRP3 inflammasome and necroptosis

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