| Yazarlar (10) |
Mustafa Bodu
Prairie View A&M University, United States |
Doç. Dr. Mustafa HİTİT
Kastamonu Üniversitesi, Türkiye |
|
Ayse Sari
Necmettin Erbakan Üniversitesi, Turkey |
|
Mesut Kirbas
Bahri Dagdas International Agricultural Research Institute, Turkey |
|
Bulent Bulbul
Dokuz Eylül Üniversitesi, Turkey |
|
Mehmet Bozkurt Ataman
Selçuk Üniversitesi, Turkey |
|
Mustafa Numan Bucak
Selçuk Üniversitesi, Turkey |
|
John Parrish
University of Wisconsin-Madison, United States |
|
Abdullah Kaya
Selçuk Üniversitesi, Turkey |
|
Erdogan Memili
Prairie View A&M University, United States |
| Özet |
| The aim of this study is to analyze DNA integrity, shape morphology, and membrane integrity in sperm from low-fertility (LF) and adequate- or normal-fertility (AF) rams. Various sperm evaluation methods such as sperm chromatin dispersion assay, Fourier harmonic amplitude (FHA) analysis, and other image analysis of morphometric parameters were used. An additional aim is to employ new statistical models with high reliability to predict ram fertility based on sperm head morphology parameters. Fresh semen was collected from 41 AF (conception rate 95.1% ± 0.6%) and 27 LF (conception rate 79.7% ± 2.5%) rams using artificial vagina. Differences (p < 0.05) were observed in percent motile sperm (mean ± SEM, 64% ± 3%, 72% ± 2%), percent viable sperm (78 ± 2%, 84 ± 1%), and head and acrosome abnormalities (1.9% ± 0.4%, 3.4% ± 0.4%) between LF and AF rams. The findings of different analyses also showed that the fertility of rams is not associated with DNA fragmentation (p > 0.05). Using the FHA analysis, an average head shape of ram sperm was constructed and harmonic amplitude 2 was determined, which tended to differ between the two ram fertility groups (p = 0.059). Based on the FHA and morphometric analysis, a significant linear discriminant model was constructed (p = 0.0013), which allowed for specificity in identifying LF rams (6/9, 66.7%) and sensitivity in identifying AF rams (39/47, 83.0%). The overall error rate remained good, which was 11/56 (20%). The findings of this study suggest that sperm DNA damage might not be used to predict ram fertility and that the statistical model based on the FHA analysis can be a potential tool in predicting ram fertility. |
| Anahtar Kelimeler |
| DNA integrity | fertility | Fourier analysis | ram | sperm head |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | Frontiers in Veterinary Science |
| Dergi ISSN | 2297-1769 Wos Dergi Scopus Dergi |
| Dergi Grubu | Q1 |
| Makale Dili | İngilizce |
| Basım Tarihi | 01-2025 |
| Cilt No | 12 |
| Sayı | 1 |
| Doi Numarası | 10.3389/fvets.2025.1577004 |
| Atıf Sayıları |
