Spectrophotometric Determination of Paracetamol in Urine with Tetrahydroxycalix 4 arene as a Coupling Reagent and Preconcentration with Triton X 114 Using Cloud Point Extraction
Yazarlar (5)
Hayati Filik
Istanbul Üniversitesi, Türkiye
Istanbul Üniversitesi, Türkiye
Prof. Dr. İzzet ŞENER Pamukkale Üniversitesi, Türkiye
Sema Demirci Cekiç Istanbul Üniversitesi, Türkiye
Emine Kiliç
Ankara Üniversitesi, Türkiye
Ankara Üniversitesi, Türkiye
Reşat Apak
Istanbul Üniversitesi, Türkiye
Istanbul Üniversitesi, Türkiye
| Makale Türü |
Özgün Makale
(SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
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| Dergi Adı | Chemical and Pharmaceutical Bulletin | ||
| Dergi ISSN | 0009-2363 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI | ||
| Makale Dili | İngilizce | Basım Tarihi | 06-2006 |
| Kabul Tarihi | – | Yayınlanma Tarihi | 01-01-2006 |
| Cilt / Sayı / Sayfa | 54 / 6 / 891–896 | DOI | 10.1248/cpb.54.891 |
| Makale Linki | https://www.jstage.jst.go.jp/article/cpb/54/6/54_6_891/_pdf | ||
| Özet |
| In the present paper, conventional spectrophotometry in conjunction with cloud point extraction-preconcentration were investigated as alternative methods for paracetamol (PCT) assay in urine samples. Cloud point extraction (CPE) was employed for the preconcentration of p-aminophenol (PAP) prior to spectrophotometric determination using the non-ionic surfactant Triton X-114 (TX-114) as an extractant. The developed methods were based on acidic hydrolysis of PCT to PAP, which reacted at room temperature with 25, 26, 27, 28-tetrahydroxycalix [4] arene (CAL4) in the presence of an oxidant (KIO4) to form an blue colored product. The PAP-CAL4 blue dye formed was subsequently entrapped in the surfactant micelles of Triton X-114. Cloud point phase separation with the aid of Triton X-114 induced by addition of Na2SO4 solution was performed at room temperature as an advantage over other CPE assays requiring elevated temperatures. The 580nm-absorbance maximum of the formed product was shifted bathochromically to 590nm with CPE. The working range of 1.5—12 mg ml 1 achieved by conventional spectrophotometry was reduced down to 0.14—1.5 mg ml 1 with cloud point extraction, which was lower than those of most literature flow-through assays that also suffer from nonspecific absorption in the UV region. By preconcentrating 10 ml sample solution, a detection limit as low as 40.0 ng ml 1 was obtained after a single-step extraction, achieving a preconcentration factor of 10. The stoichiometric composition of the dye was found to be 1: 4 (PAP: CAL4). The impact of a number of parameters such as concentrations of CAL4, KIO4 … |
| Anahtar Kelimeler |
| Calix[4]arene | Cloud point extraction | P-aminophenol | Paracetamol determination | Spectrophotometry | Urine |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Google Scholar | 55 |
| Web of Science | 36 |
| Scopus | 35 |