| Yazarlar (7) |
Sultan Sacide Gelen
Ege Üniversitesi, Türkiye |
|
Burak Gokce
Ege University Medical School, Türkiye |
Prof. Dr. İdris YAZGAN
Kastamonu Üniversitesi, Türkiye |
|
Ecem Saygili
Izmir Democracy University, Türkiye |
|
Haydar Soydaner Karakuş
Ege University Medical School, Türkiye |
|
Tuncay Goksel
Ege University Medical School, Türkiye |
|
Dilek Odaci
Ege Üniversitesi, Türkiye |
| Özet |
| C-reactive protein (CRP) is a member of the acute phase reactants. CRP is generally used as a biomarker of inflammation and tissue injury. Here, CRP detection is performed in pleural fluid caused by pleural effusion. For this aim, Anti-CRP was conjugated on silver nanoparticle (AgNP)-modified electrodes to prepare immunosensors. To carry out AgNP electrodeposition, a novel ligand, mannose 4-aminophenyl L-alanine (Man-4APA), was synthesized and characterized using NMR and MS analysis. Then, AgNPs were synthesized in the presence of Man-4APA. After that, AgNPs/P (Man-4APA) was formed by electrodeposition on the electrodes. The success of the coating of the surfaces with AgNPs/P (Man-4APA) was confirmed using SEM and EDS analysis. Covalent conjugation was carried out between the amine groups of AgNPs/P (Man-4APA) and the carboxyl groups of Anti-CRP. The formation of the obtained amide bond was examined using XPS analysis. Using the designed AgNPs/P (Man-4APA)/Anti-CRP, the linear range for CRP was 0.5–100 ng/mL with a LOD of 0.38 ng/mL. After testing the effect of some potential interfering compounds on the current signal of AgNPs/P (Man-4APA)/Anti-CRP, the determination of CRP in pleural fluid collected from patients with pleural effusion was carried out successfully. |
| Anahtar Kelimeler |
| CRP | Glyconanoparticles | Nanobiotechnology | Pleural fluid | Silver nanoparticles |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale |
| Dergi Adı | Talanta |
| Dergi ISSN | 0039-9140 Wos Dergi Scopus Dergi |
| Dergi Grubu | Q1 |
| Makale Dili | İngilizce |
| Basım Tarihi | 02-2026 |
| Cilt No | 298 |
| Sayı | 1 |
| Doi Numarası | 10.1016/j.talanta.2025.128931 |
| Atıf Sayıları |
