Immunosensing of C-reactive protein in pleural fluid using electrodeposited silver nanoparticles
Yazarlar (7)
Sultan Sacide Gelen
Ege Üniversitesi, Türkiye
Ege Üniversitesi, Türkiye
Burak Gokce Ege University Medical School, Türkiye
Prof. Dr. İdris YAZGAN Kastamonu Üniversitesi, Türkiye
Ecem Saygili Izmir Democracy University, Türkiye
Haydar Soydaner Karakuş Ege University Medical School, Türkiye
Tuncay Goksel Ege University Medical School, Türkiye
Prof. Dr. Dilek Odaci Ege Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Talanta (Q1) | ||
| Dergi ISSN | 0039-9140 Wos Dergi Scopus Dergi | ||
| Makale Dili | İngilizce | Basım Tarihi | 02-2026 |
| Cilt / Sayı / Sayfa | 298 / 1 / – | DOI | 10.1016/j.talanta.2025.128931 |
| Özet |
| C-reactive protein (CRP) is a member of the acute phase reactants. CRP is generally used as a biomarker of inflammation and tissue injury. Here, CRP detection is performed in pleural fluid caused by pleural effusion. For this aim, Anti-CRP was conjugated on silver nanoparticle (AgNP)-modified electrodes to prepare immunosensors. To carry out AgNP electrodeposition, a novel ligand, mannose 4-aminophenyl L-alanine (Man-4APA), was synthesized and characterized using NMR and MS analysis. Then, AgNPs were synthesized in the presence of Man-4APA. After that, AgNPs/P (Man-4APA) was formed by electrodeposition on the electrodes. The success of the coating of the surfaces with AgNPs/P (Man-4APA) was confirmed using SEM and EDS analysis. Covalent conjugation was carried out between the amine groups of AgNPs/P (Man-4APA) and the carboxyl groups of Anti-CRP. The formation of the obtained amide bond was examined using XPS analysis. Using the designed AgNPs/P (Man-4APA)/Anti-CRP, the linear range for CRP was 0.5–100 ng/mL with a LOD of 0.38 ng/mL. After testing the effect of some potential interfering compounds on the current signal of AgNPs/P (Man-4APA)/Anti-CRP, the determination of CRP in pleural fluid collected from patients with pleural effusion was carried out successfully. |
| Anahtar Kelimeler |
| CRP | Glyconanoparticles | Nanobiotechnology | Pleural fluid | Silver nanoparticles |
Science Direct
Immunosensing of C-reactive protein in pleural fluid using electrodeposited silver nanoparticles
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Scopus | 2 |