Isolating cancer stem cells in breast cancer models: a study of CD marker expression and Rhodamine 123 as predictors of chemoresistance
Yazarlar (7)
Öğr. Gör. Nilgün Okşak Harran Üniversitesi, Türkiye
Işık Neslişah Korkut
Istanbul Üniversitesi, Türkiye
Istanbul Üniversitesi, Türkiye
Ayşe Erol-Bozkurt Istanbul Üniversitesi, Türkiye
Ferdane Danışman-Kalındemirtaş
Erzincan Binali Yıldırım Üniversitesi, Türkiye
Erzincan Binali Yıldırım Üniversitesi, Türkiye
Doç. Dr. Dilşad ÖZERKAN Kastamonu Üniversitesi, Türkiye
Öğr. Gör. Mediha Süleymanoğlu Istanbul Üniversitesi, Türkiye
Serap Erdem-Kuruca İstanbul Atlas Üniversitesi, Türkiye
| Makale Türü |
Özgün Makale
(SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
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| Dergi Adı | Cytotechnology (Q3) | ||
| Dergi ISSN | 0920-9069 Dergi Bilgileri (2026) | ||
| Dergi Tarandığı Indeksler | SCI | ||
| Makale Dili | Türkçe | Basım Tarihi | 01-2026 |
| Kabul Tarihi | 02-01-2026 | Yayınlanma Tarihi | 27-01-2026 |
| Cilt / Sayı / Sayfa | 78 / 1 / 35–0 | DOI | 10.1007/s10616-026-00892-1 |
| Makale Linki | https://pmc.ncbi.nlm.nih.gov/articles/PMC12847529/ | ||
| UAK Araştırma Alanları |
Hücre Biyolojisi
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| Özet |
| It is believed that CSCs, which are resistant to chemotherapy and radiation, develop in certain situations, stay in the tissue niche after treatment, and cause disease recurrence. Targeting these cells, which are thought to be chemoresistant, may enable treatment to eliminate metastasis or recurrence. For this reason, we aimed to isolate CSCs from various breast cancer cell lines using a drug selection method in this study and then identify them via flow cytometry and fluorescent staining. MDA-123, MCF-7, and 4T1 were used as in vitro models, with HUVEC as controls. After isolation of stem cells from these cells by incubation with 5-fluorouracil (5-FU) and cisplatin (Cis), CSCs were identified by flow cytometry using CD24/CD44/CD133/EPCAM by assessing the rhodamine 123 (Rho 123). The results showed that 5-FU and Cis reduced the CD24 receptor in both cancer and normal cells, while the others showed … |
| Anahtar Kelimeler |
| Cancer stem-like cell | CD133 | CD44-CD24 | EpCAM | Rhodamine 123 |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Web of Science | 1 |
| Scopus | 1 |
| Google Scholar | 1 |