Thermodynamic Study of the Binding of Mercury Ion to Human Growth Hormone at Different Temperatures
Yazarlar (9)
E. Tazikeh Lemeski Islamic Azad University, Science And Research Branch, İran
G. Rezaei Behbehani Islamic Azad University, Takestan Branch, İran
A. A. Saboury University Of Tehran, İran
Prof. Dr. Majıd MONAJJEMI Islamic Azad University, Science And Research Branch, İran
R. Zafar Mehrabian
Islamic Azad University, Gorgan Branch, İran
Islamic Azad University, Gorgan Branch, İran
M. Ahmadi Golsefidi Islamic Azad University, Gorgan Branch, İran
H. Rajabzadeh
Islamic Azad University, Dezful Branch, İran
Islamic Azad University, Dezful Branch, İran
M. T. Baei
Islamic Azad University, İran
Islamic Azad University, İran
S. Hasanzadeh Islamic Azad University, Gorgan Branch, İran
| Makale Türü | Özgün Makale (ESCI dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Journal of Solution Chemistry | ||
| Dergi ISSN | 0095-9782 Wos Dergi Scopus Dergi | ||
| Makale Dili | İngilizce | Basım Tarihi | 04-2011 |
| Cilt / Sayı / Sayfa | 40 / 4 / 575–586 | DOI | 10.1007/s10953-011-9668-4 |
| Makale Linki | http://www.scopus.com/inward/record.url?eid=2-s2.0-79958048253&partnerID=MN8TOARS | ||
| Özet |
| The interaction of human growth hormone (hGH) with the divalent mercury ion was studied by isothermal titration calorimetry at two temperatures of 27°C and 37°C in aqueous solutions. We found that there is a set of two identical and non-interacting binding sites for Hg2+ ions. The intrinsic dissociation equilibrium constant and the molar enthalpy of binding are 4.2 mmol·L-1 and -14.8 kJ·mol-1 at 27°C and 5.1 mmol·L-1 and -14.2 kJ·mol-1 at 37°C, respectively. The results obtained indicate that the stability of the protein increases due to the binding of mercury ions using the extended solvation theory. © 2011 Springer Science+Business Media, LLC. |
| Anahtar Kelimeler |
| Human growth hormone (hGH) | Isothermal titration calorimetry (ITC) | Mercury ion | Metal binding | Solvation model |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Web of Science | 6 |
| Scopus | 8 |