A CALORIMETRIC STUDY ON THE INTERACTION OF ZINC ION WITH HUMAN GROWTH HORMONE
Yazarlar (5)
E. Tazikeh Islamic Azad University, Science And Research Branch, İran
A. A. Saboury University Of Tehran, İran
G. Rezaei-Behbehani Imam Khomeini International University, İran
A. A. Moosavi-Movahedi University Of Tehran, İran
Prof. Dr. Majıd MONAJJEMI Islamic Azad University, Science And Research Branch, İran
| Makale Türü | Özgün Makale (ESCI dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | Iranian Journal of Science and Technology Transaction A Science | ||
| Dergi ISSN | 1028-6276 Wos Dergi Scopus Dergi | ||
| Makale Dili | İngilizce | Basım Tarihi | 01-2009 |
| Cilt / Sayı / Sayfa | 33 / 4 / 317–327 | DOI | – |
| Makale Linki | http://www.scopus.com/inward/record.url?eid=2-s2.0-78651510772&partnerID=MN8TOARS | ||
| Özet |
| A thermodynamic study on the interaction between zinc ion (Zn2+) and human growth hormone (hGH) was studied at two temperatures of 27°C and 37°C in aqueous solution using an isothermal titration calorimetry. It was found that there is a set of three identical and non-interacting binding sites for Zn2+ ions. The intrinsic dissociation equilibrium constant and the molar enthalpy of binding are 1.54 mM and -17.6 Kj mol-1 at 27°C and 1.93 mM and -17.1 kJ mol-1 at 37°C, respectively. To reproduce the binding parameters of metal ion-hormone interaction over the whole range of Zn2+ concentrations a solvation theory was applied. The binding parameters deduced from the solvation model were attributed to the structural change of hGH and its biological activity due to the metal ion interaction. © Shiraz University. |
| Anahtar Kelimeler |
| Biothermodynamics | Enthalpy of binding | Human growth hormone (HGH) | Interaction | Intrinsic equilibrium constant | Isothermal titration calorimetry (itc) | Ligand binding | Metal binding | Solvation model | Zinc ion |
