Potential Novel Genotype of "Bopivirus B" from Sheep in Türkiye: Epidemiology and Molecular Characterization
Yazarlar (6)
Prof. Dr. Feray Alkan Ankara Üniversitesi, Türkiye
Doç. Dr. İlke Karayel Hacıoğlu Ankara Üniversitesi, Türkiye
Dr. Öğr. Üyesi Selda DURAN YELKEN Kastamonu Üniversitesi, Türkiye
Fruzsina Tóth University Of Pécs Medical School, Macaristan
Buket Pekşen
Ankara Üniversitesi, Türkiye
Ankara Üniversitesi, Türkiye
Ákos Boros University Of Pécs Medical School, Macaristan
| Makale Türü |
Özgün Makale
(SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
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| Dergi Adı | Pathogens (Q2) | ||
| Dergi ISSN | 2076-0817 Dergi Bilgileri (2026) | ||
| Dergi Tarandığı Indeksler | SCI | ||
| Makale Dili | Türkçe | Basım Tarihi | 05-2026 |
| Cilt / Sayı / Sayfa | 15 / 1 / – | DOI | 10.3390/pathogens15010052 |
| UAK Araştırma Alanları |
Veteriner Virolojisi
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| Özet |
| Various microbial agents have been found in the feces of both humans and animals, especially in newborns. While some of these agents are recognized as causing diarrhea, the role of others, specifically bopiviruses of the family Picornaviridae, in diarrhea remains uncertain. In this study, we conducted an analysis of 214 fecal samples from cattle (n = 114), sheep (n = 82), and goats (n = 18) with diarrhea, collected from farms across 17 different provinces in Türkiye. All samples were tested using RT-PCR targeting the 3D(RdRp) region of bopiviruses, and two samples from sheep (2.4%) tested positive. The 7303 nt-long complete coding sequence of Bopivirus/Sheep/KS-1M/2024/TUR and partial 3D(RdRp), VP3, and 2A-2C sequences of Bopivirus/Sheep/ANK-K30/2017/TUR were determined by additional RT-PCR, 3′RACE-PCR reactions and Sanger sequencing. Both strains show close sequence and phylogenetic relationship to members of species “Bopivirus B” of genus Bopivirus. Bopivirus/Sheep/KS-1M/2024/TUR is most closely related to a sheep Bopivirus B strain (sheep/14-73/2018/ITA) from Italy, but the phylogenetic separation, the low sequence identities and high p-distance values in VP1 to existing genotypes of “B1” and “B2” suggest that both strains could belong to novel genotypes (“B3” and “B4”) in species “Bopivirus B”, although additional closely related sequences are necessary for proper typing. |
| Anahtar Kelimeler |
| Bopivirus | phylogenetics | picornavirus | sheep | Türkiye |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları | |
| Scopus | 1 |