Using high hydrostatic pressure as an abiotic elicitor strategy for improving capsaicin production in free and immobilized cell suspension cultures of Capsicum annuum L.
Yazarlar (4)
Prof. Dr. Cemil İşlek Niğde Ömer Halisdemir Üniversitesi, Türkiye
Prof. Dr. Esra Koç Ankara Üniversitesi, Türkiye
Prof. Dr. Ergin Murat ALTUNER Kastamonu Üniversitesi, Türkiye
Prof. Dr. Hami Alpas Orta Doğu Teknik Üniversitesi, Türkiye
Makale Türü Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale)
Dergi Adı High Pressure Research (Q3)
Dergi ISSN 0895-7959 Wos Dergi Scopus Dergi
Dergi Tarandığı Indeksler SCI
Makale Dili Türkçe Basım Tarihi 01-2021
Kabul Tarihi Yayınlanma Tarihi 24-03-2021
Cilt / Sayı / Sayfa 41 / 2 / 198–208 DOI 10.1080/08957959.2021.1903458
Makale Linki https://www.tandfonline.com/doi/full/10.1080/08957959.2021.1903458
UAK Araştırma Alanları
Biyokimya
Özet
In this study, the effects of immobilization and high hydrostatic pressure (HHP) on capsaicin production in cell suspension culture of pepper seeds at different application times were investigated. Callus cultures were obtained from in vitro germinated hypocotyl explants of pepper seedlings and cell suspensions were prepared from calluses. Immobilized cell suspension cultures with calcium alginate and free cell suspension cultures were obtained by using these cell suspensions. 50, 100, 200, 300 and 400 MPa were applied to both free and immobilized cell suspensions as an elicitor. When all the results were compared, the highest amount of capsaicin was achieved in immobilized cell suspensions on the tenth day as 293.187 µg/g f.w at 400 MPa pressure. As a result, it can be concluded that the combined application of immobilization and elicitor (HHP) caused significant increases (p < .05) in the amount of …
Anahtar Kelimeler
capsaicin | Capsicum annuum | elicitor | high hydrostatic pressure (HHP) | immobilization