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Using high hydrostatic pressure as an abiotic elicitor strategy for improving capsaicin production in free and immobilized cell suspension cultures of Capsicum annuum L.      
Yazarlar
Cemil İşlek
Niğde Ömer Halisdemir Üniversitesi, Türkiye
Esra Koç
Ankara Üniversitesi, Türkiye
Prof. Dr. Ergin Murat ALTUNER
Kastamonu Üniversitesi, Türkiye
Hami Alpas
Orta Doğu Teknik Üniversitesi, Türkiye
Özet
In this study, the effects of immobilization and high hydrostatic pressure (HHP) on capsaicin production in cell suspension culture of pepper seeds at different application times were investigated. Callus cultures were obtained from in vitro germinated hypocotyl explants of pepper seedlings and cell suspensions were prepared from calluses. Immobilized cell suspension cultures with calcium alginate and free cell suspension cultures were obtained by using these cell suspensions. 50, 100, 200, 300 and 400 MPa were applied to both free and immobilized cell suspensions as an elicitor. When all the results were compared, the highest amount of capsaicin was achieved in immobilized cell suspensions on the tenth day as 293.187 µg/g f.w at 400 MPa pressure. As a result, it can be concluded that the combined application of immobilization and elicitor (HHP) caused significant increases (p <.05) in the amount of capsaicin.
Anahtar Kelimeler
capsaicin | Capsicum annuum | elicitor | high hydrostatic pressure (HHP) | immobilization
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale
Dergi Adı HIGH PRESSURE RESEARCH
Dergi ISSN 0895-7959
Dergi Tarandığı Indeksler SCI-Expanded
Dergi Grubu Q3
Makale Dili Türkçe
Basım Tarihi 04-2021
Cilt No 41
Sayı 2
Sayfalar 198 / 208
Doi Numarası 10.1080/08957959.2021.1903458
Makale Linki https://www.tandfonline.com/doi/full/10.1080/08957959.2021.1903458