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Effect of high hydrostatic pressure on physicochemical and biochemical properties of milk      
Yazarlar
Prof. Dr. Ergin Murat ALTUNER Prof. Dr. Ergin Murat ALTUNER
Kastamonu Üniversitesi, Türkiye
Hami Alpas
Orta Doğu Teknik Üniversitesi, Türkiye
Yaşar Kemal Erdem
Hacettepe Üniversitesi, Türkiye
Tahsin Faruk Bozoğlu
Orta Doğu Teknik Üniversitesi, Türkiye
Özet
Interest in high hydrostatic pressure (HHP) applications on milk and dairy products has recently increased as HHP offers a new technology for food preservation to the food industry. Although HHP-induced microbial destruction, rennet or acid coagulation of milk and increase in cheese yield has been reported, the actual effect of HHP application on milk constituents still remains to be unexplained. Therefore, we have analyzed the effect of HHP on physicochemical and biochemical properties such as turbidity, pH and especially protein micelle surface hydrophobicity of milk proteins. To serve for this purpose, milk samples with different fat contents were pressurized from 110 to 440 MPa at 25 °C for 10 and 20 min. Turbidity decreased with pressure increase and there was a slight change in pH. In order to measure the extent of exposure of hydrophobic groups of proteins to HHP, the method described by Bonomi et al. [1], based on use of a fluorescent probe, was utilized. In the light of the results obtained, it can be concluded that HHP has an effect on non-covalent interactions and especially hydrophobic bonds in milk. As the pressure is increased from 110 to 440 MPa, the micelles possibly decompose into sub-micelles and the embedded hydrophobic areas inside these micelles re-position in such a way that they can readily interfere with the fluorescent marker, ANS. These results may lead to practical applications of HHP treatment in the dairy industry to produce microbiologically safe, minimally processed products with high nutritional and sensory quality and novel texture.
Anahtar Kelimeler
ANS | High pressure | Micelles | Milk | Surface hydrophobicity
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale
Dergi Adı EUROPEAN FOOD RESEARCH AND TECHNOLOGY
Dergi ISSN 1438-2377
Dergi Tarandığı Indeksler SCI-Expanded
Makale Dili İngilizce
Basım Tarihi 02-2006
Cilt No 222
Sayı 3
Sayfalar 392 / 396
Doi Numarası 10.1007/s00217-005-0072-4