The Field Evaluation of the Diagnostic Performance of a Commercial Pregnancy-Associated Glycoprotein-Based Lateral Flow Assay for Early Pregnancy Detection in Ewes
Yazarlar (1)
Doç. Dr. Gökhan UYANIK Kastamonu Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (Uluslararası alan indekslerindeki dergilerde yayınlanan tam makale) | ||
| Dergi Adı | The Veterinary Journal | ||
| Makale Dili | – | Basım Tarihi | 02-2026 |
| Cilt / Sayı / Sayfa | 0 / 0 / 106584–0 | DOI | – |
| Makale Linki | https://www.sciencedirect.com/science/article/pii/S1090023326000407 | ||
| UAK Araştırma Alanları |
Fen Bilgisi Eğitimi
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| Özet |
| The present study aimed to determine the diagnostic accuracy of the commercial Alertys OnFarm Pregnancy Test (AOPT) based on pregnancy-associated glycoprotein (PAG) for early pregnancy diagnosis in ewes.A total of 100 multiparous Awassi ewes were synchronised for estrus and mated during the non-breeding season. Whole blood samples were collected from the jugular vein of all ewes on days 21, 28, and 35 after mating. The AOPT was first performed on the collected whole blood samples. Plasma PAG concentrations were then determined by Enzyme-Linked Immunosorbent Assay (ELISA). Pregnancy diagnosis was made on the same days by transrectal ultrasonography (USG), which was accepted as the reference standard. The sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and accuracy of AOPT compared to USG on days 21, 28, and 35 after mating were … |
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