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Identification of Genotype and Allelic Frequencies of Vitamin D Receptor Gene Taq1 Polymorphisms in T1DM Patients from Turkey   
Yazarlar (2)
Cingilli Vural Hasibe
Dr. Öğr. Üyesi Orçun İŞLER ÖZGÜN Dr. Öğr. Üyesi Orçun İŞLER ÖZGÜN
Türkiye
Devamını Göster
Özet
Changes in the Deoksiribonucleic acid (DNA), or polymorphisms, of the Vitamin D Receptor (VDR) cause the protein to bind more or less tightly to 1.25 Hidroksil (OH). The tighter that vitamin D binds, the stronger and longer lasting the metabolic changes are. Some of the different polymorphisms of the VDR have been associated with an increased risk for Diabetes Mellitus. This study suggests that while Taq1 polymorphisms may be functionally different it may also play a role in serum levels. Therefore, in this study, we selected TT allele of VDR gene has been associated with higher Diabetes Mellitus risk for study and investigated young adults or 100 patients with T1DM (50 women, 50 men) and 120 healthy subjects. The Polymerase chain reaction (PCR) was used for amplification of a 200 bp fragment of the VDR gene. One study found that TT genotype are over-represented in T1DM patients and those with the TT allele had a 3 fold increase in T1DM risk. In addition, the aim of the present study was to adapt PCR amplification, the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and the most effective DNA isolation method. The results indicate that the Taq 1 polymorphism in the VDR gene plays a significant role in protection against T1DM. Consequently, We found an association between VDR polymorphism (Taq1) and T1DM in Turk population. Abbrevations Diabetes Mellitus (DM); Single Nucleotid Polymorphism (SNP); Polymerase Chain Reaction (PCR); Vitamin D Receptor (VDR), Restriction Fragment Lenght Polymorphism (RFLP).
Anahtar Kelimeler
Makale Türü Özgün Makale
Makale Alt Türü Diğer hakemli uluslarası dergilerde yayınlanan tam makale
Dergi Adı Journal of Applied Biological Science
Dergi ISSN 1307-1130 Scopus Dergi
Dergi Tarandığı Indeksler Index Copernicus
Makale Dili İngilizce
Basım Tarihi 06-2011
Cilt No 5
Sayı 3
Sayfalar 39 / 42
Makale Linki https://www.jabsonline.org/index.php/jabs/article/view/259
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