Evaluation of protective effects of gossypin against hydrogen peroxide damage in L929 fibroblast cells
Yazarlar (1)
| Makale Türü | Özgün Makale (Uluslararası alan indekslerindeki dergilerde yayınlanan tam makale) | ||
| Dergi Adı | Kafkas Journal of Medical Sciences | ||
| Makale Dili | – | Basım Tarihi | 01-2020 |
| Cilt / Sayı / Sayfa | 10 / 0 / 15–23 | DOI | – |
| Makale Linki | https://elibrary.ru/item.asp?id=77872597 | ||
| UAK Araştırma Alanları |
Tıbbi Farmakoloji
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| Özet |
| Aim In this study, we evaluated the capacity of Gossypin in H 2 O 2induced oxidative stress and wound healing model in mouse L929 fibroblast cells. Material and Method In vitro wound healing activity, cell viability and cytotoxicity indexes of Gossypin (5-200 µg/mL) and H 2 O 2 (0.3-0.9 mM) were evaluated in the mouse fibroblast cell line L929 using the MTT assay. Migration test was performed as an in vitro wound healing test model. The concentration of H 2 O 2 (0.5 mM) was used to evaluate the antioxidant activity. SOD activity and MDA level were determined. We also analyzed IL-1β and TNF-α mRNA expression levels in L929 cells using real-time PCR to assess whether Gossypin doses could reduce H2O2-induced damage. Results H 2 O 2 (0.3-0.9 mM) was dose-dependent and gossypin (5-200 µg/mL) inhibited proliferation to L929 cells after 75 µg/mL. 25 and 50 µg/mL doses of Gossypin protected L929 … |
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