Inoculum Standardisation: Ensuring Reliable Antimicrobial Study Outcomes
Yazarlar (1)
Prof. Dr. Ergin Murat ALTUNER Kastamonu Üniversitesi, Türkiye
| Makale Türü |
Özgün Makale
(Uluslararası alan indekslerindeki dergilerde yayınlanan tam makale)
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| Dergi Adı | altuner. me | ||
| Makale Dili | – | Basım Tarihi | 05-2025 |
| Cilt / Sayı / Sayfa | 5 / 29 / 1–5 | DOI | – |
| Makale Linki | http://www.altuner.me/Blog1.php | ||
| UAK Araştırma Alanları |
Biyokimya
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| Özet |
| Accurate inoculum standardisation is fundamental for reliable outcomes in anti-infective studies, including antimicrobial susceptibility testing (AST). McFarland turbidity standards, particularly the 0.5 McFarland standard (approximating 1.5 x 10⁸ CFU/mL for bacteria), are widely employed for this purpose. These standards are prepared by reacting barium chloride with sulphuric acid to produce a barium sulphate precipitate of defined turbidity. This text details their preparation, including quality control via spectrophotometry, proper usage involving visual comparison against a Wickerham card, and critical storage conditions (room temperature or refrigerated, protected from light) to maintain their integrity and typical 6-month shelf-life for laboratory-prepared versions. Furthermore, it touches upon alternative standardisation methods, such as spectrophotometry and automated systems, and discusses the limitations inherent to McFarland standards, including operator subjectivity and precipitate instability. Ultimately, meticulous inoculum standardisation ensures the generation of reproducible and comparable data, crucial for both clinical diagnostics and research in microbiology. |
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