Monitoring embryological development and determination of survival ratios after short-term storage of rainbow trout (Oncorhynchus mykiss) eggs using lemon balm (Melissa …
Yazarlar (1)
Dr. Öğr. Üyesi Rahmi Can ÖZDEMİR Kastamonu Üniversitesi, Türkiye
Makale Türü Özgün Makale (Uluslararası alan indekslerindeki dergilerde yayınlanan tam makale)
Dergi Adı
Makale Dili Basım Tarihi 01-2018
Cilt / Sayı / Sayfa 4 / 2 / 14–19 DOI 10.5555/20203175903
Makale Linki https://www.cabidigitallibrary.org/doi/full/10.5555/20203175903
UAK Araştırma Alanları
Su Ürünleri Yetiştiriciliği
Özet
In this study, fertilization ability of eggs, obtained by artificial stripping from female rainbow trout, after short term storage within ovarian fluid (4°C) was determined at different times. This experiment was conducted in triplicates. Seven different experimental groups were established and included 0. Min, 1. h, 2. h, 3. h, 4. h, 5. h and 6. h after artificial stripping. Besides ovarian fluid, methanolic extracts of lemon balm (Melissa officinalis) and coriander (Coriandrum sativum) were used in the 5. and 6. h groups as natural immune booster. Every experimental groups were composed of 35-40g (~250) eggs. A 0.3 NaCl solution was used in sperm activation and fertilization procedure was performed. Sperm was collected from male rainbow trout and first checked for motility was determined under a light microscope before fertilization. Percentage of motilite and motility time of each sperm sample was controlled 3 time …
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