| Makale Türü |
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| Dergi Adı | PQDT-Global | ||
| Makale Dili | – | Basım Tarihi | 01-2013 |
| Makale Linki | https://scholar.google.com/scholar?cluster=12590415047376094097&hl=en&oi=scholarr | ||
| UAK Araştırma Alanları |
Ayırma İşlemleri
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| Özet |
| The scope of the study is to investigate calf thymus DNA adsorption process on solid powders such as silica, alumina and hydroxyapatite (HAP) to improve DNA solid phase extraction efficiency and to visiulize DNA adsorption by atomic force microscopy (AFM). The equilibrium and kinetics of the DNA adsorption were investigated in batch adsorption on a commercial silica gel and a synthesized silica aerogel, commercial alumina and HAP powders from aqueous DNA solution. Commercial DNA extraction kit adsorbents were also characterized and used for adsorption. Adsorbed DNA was imaged in ambient air on flat surfaces of mica, silica and alumina wafers and HAP particles coated glass surfaces and a HAP pellet surface by AFM. Effects of ambient air, nitrogen flow and freeze drying methods on DNA morphology on the releted surfaces were also investigated. |
| Anahtar Kelimeler |