Thermodynamics of the alkaline transition in phytocyanins
Yazarlar (7)
Gianantonio Battistuzzi
Marzia Bellei
Christopher Dennison
Giulia Di Rocco
Katsuko Sato
Marco Sola
| Makale Türü | Özgün Makale (Uluslararası alan indekslerindeki dergilerde yayınlanan tam makale) | ||
| Dergi Adı | JBIC Journal of Biological Inorganic Chemistry | ||
| Makale Dili | – | Basım Tarihi | 01-2007 |
| Cilt / Sayı / Sayfa | 12 / 6 / 895–900 | DOI | 10.1007/s00775-007-0245-7 |
| Makale Linki | https://link.springer.com/article/10.1007/s00775-007-0245-7 | ||
| UAK Araştırma Alanları |
Fiziksel Kimya
|
||
| Özet |
| The thermodynamics of the alkaline transition which influences the spectral and redox properties of the type 1 copper center in phytocyanins has been determined spectroscopically. The proteins investigated include Rhus vernicifera stellacyanin, cucumber basic protein and its Met89Gln variant, and umecyanin, the stellacyanin from horseradish roots, along with its Gln95Met variant. The changes in reaction enthalpy and entropy within the protein series show partial compensatory behavior. Thus, the reaction free energy change (hence the pKa value) is rather variable. This indicates that species-dependent differences in reaction thermodynamics, although containing an important contribution from changes in the hydrogen-bonding network of water molecules in the hydration sphere of the protein (which feature enthalpy–entropy compensation), are to a large extent protein-based. The data for axial ligand variants … |
| Anahtar Kelimeler |